酶抑制荧光探针法检测果蔬样品中农药残留的研究  

Determination of Pesticide Residues in Real Samples Based on Enzyme Inhibition Using a Fluorescent Probe

作　　者：金盛烨[1] 陈吉平[1] 郭荣波[1] 梁鑫淼[1] 

机构地区：[1]中国科学院大连化学物理研究所103组,辽宁大连116011

出　　处：《精细化工》2004年第12期946-949,共4页Fine Chemicals

摘　　要：基于已建立的酶抑制荧光探针流动注射分析法,通过对6种蔬菜和水果样品加标呋喃丹农药的实验,对其农药残留进行了检测,考察了该法在实际应用中的可靠性,研究了基质pH对检测的影响。获得呋喃丹的回收率在83 6%～110 9%,RSD在1 9%～8 1%,证明该法可以有效地应用在实际果蔬样品的检测中。样品酸度对检测有一定影响,对酸度较高的样品,通过调节基质的pH=7 5即可获得满意的检测结果。A method, which is based on enzyme inhibition using a fluorescence probe,was applied to the determination of carbofuran in spiked vegetable and fruit samples.Satisfactory recovery values between 83.6%～110.9% and RSDs between 1.9%～8.1% were achieved.The influences of pH of samples on the detection of carbofuran are discussed.For the detection of the samples with low pH values,the pH need to be adjusted to higher ones to obtaining satisfactory detection results.

关 键 词：酶抑制 荧光探针 流动注射分析 农药 

分 类 号：TQ450.26[化学工程—农药化工]