基于Au纳米通道膜的生物分离新方法在蛋白质分离中的应用  

A New Biochemical Method for Protein Separation Based on Gold Nanotubule Membrane

作　　者：黄杉生[1] 殷月芬[1] 王柯敏[1] 何晓晓[1] 钟桐生[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室

出　　处：《高等学校化学学报》2004年第12期2238-2241,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金重点项目 (批准号 :2 0 13 5 0 10 );国家重点基础研究发展规划项目 (批准号 :2 0 0 2 CB5 13 10 0 -10 );国家科技攻关计划项目 (批准号 :2 0 0 3 BA3 3 10 A16);科技部国际合作重点项目 (批准号 :2 0 0 3 DF 0 0 0 0 3 9)资助

摘　　要：采用化学沉积法将 Au沉积到聚碳酸酯滤膜 ( PC-Mem)上 ,制备了直径为 5 5 nm左右的 Au纳米通道膜 ( Au-Mem) .以牛血清白蛋白 ( BSA)和免疫球蛋白 G( Ig G)抗体为模型分子 ,研究了 Au-Mem以及分别以半胱氨酸和硫氰酸胍修饰的 Au-Mem( Cys-Au-Mem,Gua-Au-Mem)对模型分子的分离特性 .由于硫氰酸胍具有一定的亲水作用和蛋白质变性作用 ,BSA在 Gua-Au-Mem上的迁移速率比在 Au-Mem上增加了 3 0～ 5 0倍 ,而修饰膜对 Ig G的迁移速率无明显影响 .在 p H=4.5时分离了 BSA和 Ig G的混合溶液 .Gua-Au-Mem和 Cys-Au-Mem对混合溶液的分离度分别为 3 1 .6和 2 3 .1 ,表明经修饰的Gold nanotubule membranes with about 55 nm of diameter were prepared by chemical deposition of gold on polycarbonate templates membrane. After being modified by cysteine and guanide thiocyanate, the nanomembranes were studied with BSA and IgG as the model molecules. The results show that guanide thiocyanate facilitated transporting of BSA by 30 to 50 times because of hydrophilic and denaturalization effects whereas IgG almost retained its′ transporting speed. Then the separation processes were carried out and separation degrees of 31.6 and 23.1 were obtained for cysteine and guanide thiocyanate modified gold nanotubules, respectively at pH 4.5. The results indicate that gold nanotubule membranes has a good separating capability for protein.

关 键 词：Au纳米通道膜 蛋白质 迁移 分离 

分 类 号：O657[理学—分析化学]