以赖氨酸为配基的内毒素吸附剂的研究  

Hemperfusion Adsorbents With Lysine as Ligand for Removing of Endotoxin

作　　者：刘涛[1] 侯光辉[1] 俞玫[1] 袁直[1] 刘斌[2] 赵承梅[2] 沈彬[2] 王燕铭[3] 王深琪[3] 

机构地区：[1]南开大学高分子化学研究所吸附分离功能高分子材料国家重点实验室,天津300071 [2]天津医学高等专科学校,天津300052 [3]南开大学生物活性材料教育部重点实验室,天津300071

出　　处：《高等学校化学学报》2004年第12期2284-2286,共3页Chemical Journal of Chinese Universities

基　　金：国家"九七三"计划项目 (批准号 :G19990 64 70 7);国家自然科学基金 (批准号 :5 0 3 73 0 2 1);教育部博士学科点基金 (批准号 :2 0 0 3 0 0 5 5 0 3 8);南开大学科技创新基金资助

摘　　要：Endotoxin is lipopolysaccharide(LPS) derived from the cell membrane of gram-negative bacteria. The concentration of endotoxin in endotoxemia patients′ blood increases quickly and leads to severe sepsis, leading to severe hypertension, cardiovascular continues to have a high failure and death, which is a major cause of death in patients and continues to have a high mortality despite appropriate surgery, potent antibiotic and intensive supportive therapy was conducted. Removal of endotoxin duly and effectually from patients′ blood is very important in clinic. Hemoperfusion can clear toxins from patient′s blood by adsorbent, not only small molecular toxins but also large ones in time. In this paper, the hemoperfusion adsorbent for removing endotoxin was prepared. The physical and chemical parameter of the adsorbent is investigated and the surface structure of adsorbent was investigated through ESEM. The influence of weights of adsorbents and adsorbing time on adsorption ability was also studied. The results showed that the adsorbent with lysine as the ligand had good affinity to endotoxin and the best adsorption capacity of it was 253.1 EU/g when the initial concentration is 10 EU/mL. The blood compatibility of the adsorbent was also studied and the results indicated that the adsorbent has clinic application foreground.Endotoxin is lipopolysaccharide(LPS) derived from the cell membrane of gram-negative bacteria. The concentration of endotoxin in endotoxemia patients′ blood increases quickly and leads to severe sepsis, leading to severe hypertension, cardiovascular continues to have a high failure and death, which is a major cause of death in patients and continues to have a high mortality despite appropriate surgery, potent antibiotic and intensive supportive therapy was conducted. Removal of endotoxin duly and effectually from patients′ blood is very important in clinic. Hemoperfusion can clear toxins from patient′s blood by adsorbent, not only small molecular toxins but also large ones in time. In this paper, the hemoperfusion adsorbent for removing endotoxin was prepared. The physical and chemical parameter of the adsorbent is investigated and the surface structure of adsorbent was investigated through ESEM. The influence of weights of adsorbents and adsorbing time on adsorption ability was also studied. The results showed that the adsorbent with lysine as the ligand had good affinity to endotoxin and the best adsorption capacity of it was 253.1 EU/g when the initial concentration is 10 EU/mL. The blood compatibility of the adsorbent was also studied and the results indicated that the adsorbent has clinic application foreground.

关 键 词：内毒素血症 内毒素 吸附剂 全血灌流 

分 类 号：Q592.1[生物学—生物化学] Q592.2