含核酸碱基混合脂质体的制备与膜内分子识别  

Preparation of Phosphatidylcholine/Cationic Amphiphilic Coliposomes and Molecular Recognition in the Bilayer Membranes

作　　者：宋立明[1] 凌星[1] 李国文[1] 

机构地区：[1]吉林大学超分子结构与材料教育部重点实验室,长春130023

出　　处：《高等学校化学学报》2004年第12期2356-2358,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 2 740 15 );吉林大学创新基金资助

摘　　要：An amphiphile, 9-octanyl adenine trimethylammonium bromide(OATMAB amphiphile 3), was synthesized as the receptor. The coliposomes were prepared from phosphatidylcholine and 9-octyl adenine trimethylammonium bromide by sonication of CHCl 3-cast films of amphiphiles mixtures [n(PC)∶ n(MOATMAB)=10∶1] in doubly distilled water with a bath-type sonicator at 80 W for 3 h. As thymine was added into PC/MAOTMAB coliposomal solution, the absorbability of thymine at 263 nm decreased slowly with time change from 0 to 72 h. The UV-Vis spectroscopic behavior of the thymine in PC/OATMAB/thymine coliposomes(molar ratio=10∶1∶1) was slowly decreaced with the increase of time. The onset of a hypochromic effect can be ascribed to adenine-thymine interaction. The absorption band levels off at 45.4% of its initial value and stabilizes after several weeks.An amphiphile, 9-octanyl adenine trimethylammonium bromide(OATMAB amphiphile 3), was synthesized as the receptor. The coliposomes were prepared from phosphatidylcholine and 9-octyl adenine trimethylammonium bromide by sonication of CHCl 3-cast films of amphiphiles mixtures [n(PC)∶ n(MOATMAB)=10∶1] in doubly distilled water with a bath-type sonicator at 80 W for 3 h. As thymine was added into PC/MAOTMAB coliposomal solution, the absorbability of thymine at 263 nm decreased slowly with time change from 0 to 72 h. The UV-Vis spectroscopic behavior of the thymine in PC/OATMAB/thymine coliposomes(molar ratio=10∶1∶1) was slowly decreaced with the increase of time. The onset of a hypochromic effect can be ascribed to adenine-thymine interaction. The absorption band levels off at 45.4% of its initial value and stabilizes after several weeks.

关 键 词：核酸碱基 分子识别 双亲化合物 混合脂质体 

分 类 号：O641.3[理学—物理化学]