N,N'-多亚甲基撑双二乙醇胺铜金属配合物对质粒DNA的催化水解  

The Hydrolysis of Plasmid DNA Catalyzed by Cu(Ⅱ) Complexes of N,N~-Polymethylene bis(diethanolamine) Ligands

作　　者：蒋福宾[1] 兰利琼[2] 苗琛[3] 余孝其[1] 孟祥光[1] 曾宪诚[1] 

机构地区：[1]四川大学化学学院 [2]四川大学生命科学学院,成都610064 [3]河南大学生命科学学院,河南开封475001

出　　处：《四川大学学报（自然科学版）》2004年第6期1225-1228,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(20173038;20132020;20107004)

摘　　要：作者研究了N,N′ 多亚甲基撑双二乙醇胺双核铜(Ⅱ)配合物催化DNA水解反应,初步探讨了催化质粒DNA(PBin19)水解的条件和机理.结果表明:金属配合物促进质粒DNA的水解,催化活性与配体桥联基的刚柔性有关.The catalytic hydrolysis of DNA by Cu(Ⅱ)complexes with N, N'-polymethylene bis (diethanolamine) ligands have been investigated in this paper. The condition and the mechanism of the catalytic hydrolysis of Plasmid DNA(PBin19) are investigated preliminarily. The results indicate that the complexes have successfully promoted the hydrolytic cleavage of plasmid DNA. The catalytic activities of these complexes are related to the rigidity and flexibility of the bridged ligands.

关 键 词：金属配合物 DNA水解 化学核酸酶 催化 

分 类 号：O643.32[理学—物理化学]