甘蓝型油菜(Brassica napus L.)矮秆突变位点的RAPD标记  

The RAPD Markers of Dwarf Mutation Site in Brassica napus L.

作　　者：李华鹏[1] 赵云[1] 周云涛[1] 王茂林[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2004年第6期1290-1293,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家"十五"科技攻关项目(编号:07 01 02)资助

摘　　要：50 10-mer RAPD primers were used to identify RAPD （random amplified polymorphic DNA） makers linked to the newly mutated dwarf locus in B.napus L.. Between two DNA pools of the dwarf and its wild type parent, PCR results of 7 primers showed polymorphisms and all the specific DNA fragments could only be amplified in high parent rather than in the mutant. In the F2 population of the hybrids of the wild type and the mutant, 4 of the 7 markers were identified to be linked to the mutated locus: S5910,S12610,S29390 and S47410,with a map distance of 16.6（cM）、0.0（cM）、16.6（cM） and 5.0（cM） respectively. The potential uses of these molecular makers were also discussed.50 10-mer RAPD primers were used to identify RAPD (random amplified polymorphic DNA) makers linked to the newly mutated dwarf locus in B.napus L.. Between two DNA pools of the dwarf and its wild type parent, PCR results of 7 primers showed polymorphisms and all the specific DNA fragments could only be amplified in high parent rather than in the mutant. In the F2 population of the hybrids of the wild type and the mutant, 4 of the 7 markers were identified to be linked to the mutated locus: S5_(910),S12_(610),S29_(390) and S47_(410),with a map distance of 16.6(cM)、0.0(cM)、16.6(cM) and 5.0(cM) respectively. The potential uses of these molecular makers were also discussed.

关 键 词：甘蓝型油菜 矮秆 RAPD标记 病害发生 倒伏 高秆 籽粒充实 农村产业结构调整 油菜生产 人民生活水平 

分 类 号：S565.4[农业科学—作物学] S512.1