DNA及基因组改组在代谢工程中的应用  

APPLICATIONS OF DNA SHUFFLING ANDGENOME SHUFFLING IN METABOLIC ENGINEERING

作　　者：陈涛[1] 陈洵[1] 王靖宇[1] 班睿[1] 赵学明[1] 

机构地区：[1]天津大学化工学院生物化工系,天津300072

出　　处：《化工学报》2004年第11期1753-1758,共6页CIESC Journal

基　　金：国家自然科学基金 (No 2 0 0 3 0 610 );国家重点基础研究发展规划项目 (No 2 0 0 3CB7160 0 3 )资助~~

摘　　要：代谢工程是应用分子生物学与反应工程的结合 ,已发展成为菌种改进的平台技术 .后基因组学时代的基因组学、转录组学、蛋白质组学及代谢物组学等为代谢工程的发展提供了极好的机遇 .DNA改组及基因组改组可以构建新的代谢途径、对已知或未知的代谢途径进行快速优化 ,极大地促进了代谢工程的进一步发展和应用 .DNA改组及基因组改组技术可以加深对代谢网络及其分子调节机制的理解 ,是对合理代谢设计策略的完善和补充 .本文首先分别介绍了DNA改组、基因组改组在代谢工程中的应用 ,进而展望了基于基因组改组的代谢工程方法步骤及发展方向 .Metabolic engineering is the combination of applied molecular biology and reaction engineering. It has become the platform technology for strain improvement. In the post-genomic era, genomics, transcriptomics, proteomics and metabolomics provide great opportunity for the development of metabolic engineering. In addition to new pathway construction, DNA shuffling and genome shuffling are applied to rapid optimization of known or unknown pathway, which greatly promotes the development and applications of metabolic engineering. DNA shuffling and genome shuffling methods can increase our understanding of metabolic networks and their molecular regulation mechanisms, integrating and complementing rational metabolic design strategies. This review illustrates the applications of DNA shuffling and genome shuffling in metabolic engineering and prospects the perspective and methodology in metabolic engineering by genome shuffling.

关 键 词：代谢工程 DNA改组 基因组改组 定向进化 

分 类 号：Q819[生物学—生物工程]