体外培养不同代次成纤维细胞胶原基因表达及成纤维细胞对EGF的反应性  

Expression of Collagen Gene in Different Passages of Human Fibroblasts Cultured in Vitro and Reactivity of Fibroblasts to EGF

作　　者：蔡霞[1] 高学军[2] 张鹏[1] 唐胜建[1] 杨彪炳[1] 牟少春[1] 鞠晓燕[1] 

机构地区：[1]潍坊医学院整形外科研究所 [2]潍坊医学院附属医院普外科,山东潍坊261042

出　　处：《解剖科学进展》2004年第4期295-297,共3页Progress of Anatomical Sciences

基　　金：山东省教育厅重点资助项目 (0 3K0 7)

摘　　要：目的　观察体外培养不同代次人成纤维细胞Ⅰ、Ⅲ型胶原基因的表达及对EGF刺激的反应性。方法　将正常人二倍体成纤维细胞进行连续传代培养 ,以不同代次的细胞为实验对象 ,应用RT PCR、3 H TdR掺入量测定等方法对不同代次细胞进行检测。结果　随细胞代次的增加 ,Ⅰ、Ⅲ型胶原mRNA含量逐渐降低 ,3 H TdR掺入量降低 ,EGF促进P10 培养的成纤维细胞增殖 ,对衰老成纤维细胞 (P60 )无生长增殖的调控能力。结论　为建立人成纤维细胞衰老模型提供部分实验依据。Objective To investigate the expression of collagen ge ne in different passages of human fibroblasts cultured in vitro and the reactivity of fibroblasts to epithelial growth factor (EGF). Method Fib roblasts from human normal skin were taken for successive cell culture. RT-PCR and 3H-TdR were performed. Result The expressions o f collagen Ⅰ and Ⅲ mRNAs, and content of 3H-TdR decreased gradually as t he passages of cultured human fibroblasts increased. EGF promotes the proliferat ion of P 10 cultured fibroblast instead of the P 60 cultured fibroblas t. Conclusion The present results might provide partial exper imental data for establishing human fibroblast senescent model in vitro.

关 键 词：EGF 人成纤维细胞 ^3H-TDR掺入 Ⅲ型胶原 体外培养 表达 胶原基因 连续传代 RNA 二倍体 

分 类 号：Q944.58[生物学—植物学] R619.6[医药卫生—外科学]