不同功率电凝法治疗大鼠实验性子宫内膜异位症的研究  

Electrocoagulation of experimental endometriosis at different power in rats

作　　者：牛战琴[1] 郝秋芳[1] 李莉[1] 

机构地区：[1]山西医科大学第一临床医学院妇科,太原030001

出　　处：《山西医科大学学报》2004年第6期545-547,共3页Journal of Shanxi Medical University

摘　　要：目的　探讨单极电凝治疗子宫内膜异位症的适当方法及功率。方法　将 4 0只性成熟SD雌性大鼠通过手术移植自体子宫内膜片至腹壁的方法制成大鼠子宫内膜异位症模型。选用 15W、30W、6 0W三档功率对大鼠移植灶进行单极电凝治疗 ,分别于电凝当时和 3周后取电凝移植灶行HE染色和乳酸脱氢酶活性染色 ,以观察组织电热损伤改变 ,比较 3组不同功率电凝破坏子宫内膜移植灶的效果及其对组织电热损伤的程度。同时比较先通电后电凝和先接触组织后通电电凝两种不同电凝手法对大鼠腹壁组织产生的不同电热效应。结果　三档功率皆能完全破坏移植灶活性 ,且 3周后 ,各组的移植灶皆未见活性 ,15W组与 6 0W组间的组织损伤程度有统计学差异。先通电后电凝组局部组织电热效应强而远处组织电热效应弱 ,先接触组织后通电组效果相反。结论　在对组织实施电凝治疗时 ,应先通电 ,后电凝 ;15W低功率电凝可以有效破坏有活性的异位子宫内膜腺体及间质细胞 。Objective To investigate the proper meth od and power of unipolar electrocoagulation in treatment of endometriosis. Methods Endometriosis was induced in 40 mature female rats by transplantation of endometrium on the peritoneum. Five weeks later, all the rats were laparotomized and divided into 8 groups according to different treatments and the time of examination:untreated groups (A', A groups), groups with samples taken immediately (B,C,D groups) accepted unipolar electrocoagulation with power of 15 W, 30 W and 60 W respectively and groups with samples taken three weeks later(B',C',D'groups).The operation of electrocoagulation was to switch on the electrode probe first and then coagulate the implant, or fulguration was created before coagulation. The implants were stained by hemaloxylin-eosin and lactate dehydrogenase(LDH)activity. After the electrocoagulated implants was cut off, peritoneum of B group was electrocoagulated according to the method above, while in group C, the probe was placed on the peritoneum before switching on. Both power and time were identical. Results For samples taken immediately, electrocoagulation at three different powers could destroy the activity of endometriosis, while tissue destruction increased with increasing power. For those taken three weeks later, endometriosis could also be remitted completely at different three powers and there was no LDH activity. The brown char appeared at the contacted tissue with electrode in group B, whereas only white mark in group C; the temperature of the rats back became higher significantly in group C than in group B. Conclusion The proper method of electrocoagulation is to power the electrode first, then coagulate. Electrocoagulation of endometriosis at a low power is as effective as that at a high one but more safe.

关 键 词：子宫内膜异位症 电凝术 大鼠 

分 类 号：R711.71[医药卫生—妇产科学]