抗癌中药紫龙金与HMBA对人胃癌细胞分化表型调控的比较研究  

Regulative effects of Zilongjin and HMBA on growth phenotype of human gastric cancer cells in cell cycle

作　　者：李宝园[1] 梁云燕[1] 王代树[1] 

机构地区：[1]北京市肿瘤防治研究所,北京100034

出　　处：《山西医科大学学报》2004年第6期556-558,共3页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目 (3 9480 0 2 6)

摘　　要：目的　对比研究中药紫龙金与西药环六亚甲基二己酰胺 (hexamethylenbisacelamide ,HMBA)对人胃癌MGc80 3不同周期细胞的生长和分化表型调节的共性作用。方法　采用高压氧化亚氮 (笑气 )加胸腺嘧啶脱氧核苷 (TdR)双阻断法将MGc80 3细胞分别同步化于G1 、S、G2 、M 4个时相 ,用中药紫龙金和HMBA分别处理各时相细胞。结果　紫龙金与HMBA对不同周期细胞都具有显著的增殖抑制作用 ,细胞在软琼脂上的生长态势和细胞生长抑制相似 ,但在G2 期紫龙金的作用优于HMBA。同时细胞微丝组装除M期外 ,均显著增加。结论　中药紫龙金与HMBA对人胃癌MGc80 3各时相细胞的生长分化表型的促进作用具有明显的共性特征。Objective To investigate and compare the common effect of Zilongjin, a Chinese herbal preparation ,and Hexamethylene bisacetamide(HMBA),a cell differentiation inducer usually used on growth regulation and phenotype of human gastric cancer(MGc803) cells in different phases of cell cycle. Methods MGc803 cells in monolayer cultures synchronized with nitrous oxide under high pressure were double blocked with overdosage of TdR in the four cellar phases(G 1,S,G 2,M), then collected and treated with Zilongjin and HMBA respectively. Results Both medicines could suppress the cell proliferation.So soft agar cloning growth and microfilament assembling of different phases of cells, the effect on cells of G 1 phase was the most significant one. Conclusion Zilongjin and HMBA had significant common characteristics in inducing cell differentiation of MGc803 cells in different phases of cell cycle.

关 键 词：植物制剂 紫龙金 环六亚甲基二己酰胺 腺癌 胃肿瘤 细胞周期 

分 类 号：R285.5[医药卫生—中药学]