靶向人晶状体上皮细胞免疫毫微球的制备  

Preparation of the Immunonanoparticles Targeting to Lens Epithelial Cells

作　　者：刘晓燕[1] 常津[1] 郭艳霜[1] 原续波[1] 刘新玲 李筱荣[2] 

机构地区：[1]天津大学材料学院纳米生物技术研究所,天津300072 [2]天津医科大学眼科中心,天津300070

出　　处：《高分子通报》2004年第6期63-67,共5页Polymer Bulletin

基　　金：天津市自然科学基金 (No 0 1 3 61 661 1;No 0 0 3 70 2 71 1 );国家重大基础研究 ( 973 )预研基金 2 0 0 1CCC0 1 40 0 );国家自然科学基金 ( 5 0 3 73 0 3 3 3 );天津市科委重点科技攻关项目 ( 0 2 3 1 1 1 71 1 )

摘　　要：采用偶联法使抗人晶状体上皮细胞单克隆抗体与聚乳酸载 5_氟尿嘧啶毫微球偶联 ,制备出靶向人晶状体上皮细胞的免疫毫微球。采用扫描电镜和透射电镜观察微球形貌和结构 ,用动态光散射粒径分析仪测载药毫微球粒径。ELISA法检测偶联后的抗体活性 ,间接免疫荧光法检测该免疫毫微球与晶状体上皮细胞的特异性结合能力。结果显示载药毫微球表面光滑 ,平均粒径为191 0± 0 2 0 2nm ,其载药率为 8 2 %。免疫载药毫微球中的单克隆抗体HILE6保留达到原免疫活性84 %。该免疫载药毫微球能与晶状体上皮细胞特异性结合。该实验为特异性抑制晶状体上皮细胞增殖 。The immunonanoparticle [HILE6-PLA(5-Fu)-NP] was prepared by “EDC' method cross-linking the monoclonal antibody HILE6 with PLA nanoparticles containing 5-Fu [PLA(5-Fu)-NP]. The particles were characterized for morphology and structure by scanning electronic microscopy (SEM) and transmission electronic microscopy (TEM). The particle size was determined by Particle Size Analyzer. 5-Fu drug content was also examined. ELISA method was used to evaluate the immunoreactivity. The indirect immunofluorescence method was used for evaluation of the selectivity of the INPs. We found NPs have smooth external morphology and the particle size was 191 0±0 202nm, the drug loading was 8 2%. In the immunonanoparticles, the immunological activity of antibody can reach 84% of the original HILE6. HILE6-PLA(5-Fu)-NP could bind the lens epithelia specifically. The result is very beneficial for selectively and long^time killed Lens epithelia cells for preventing posterior capsular opacification.

关 键 词：晶状体上皮细胞 载药 毫微球 靶向 并发症 免疫 单克隆抗体 制备 平均粒径 偶联 

分 类 号：TQ460[化学工程—制药化工]