人参皂甙单体Rb_3对缺血神经元胞内游离钙的影响  

The Effect of Ginsenoside Rb_3 on the Intracellular free Calcium in Ischemic Neurons

作　　者：张云峰[1] 曹茂虹[1] 柯开富[1] 

机构地区：[1]南通大学附属医院神经内科,江苏226001

出　　处：《中国交通医学杂志》2004年第6期636-638,共3页Chinese Medical JOurnal of Communications

摘　　要：目的 :研究人参的主要生物活性物质 -人参皂甙 (GS)的单体Rb3(GSRb3)对大鼠海马皮质神经元缺血损伤的保护作用。方法 :大鼠海马神经元培养后 ,分别给予模拟缺血、模拟缺血 +不同浓度的Rb3培养后 ,采用激光共聚焦技术 ,测定不同情况下大鼠海马皮质神经元胞内游离钙的情况。结果 :Rb3对大鼠模拟缺血海马神经元胞内游离钙的升高有明显抑制 ,模拟缺血 10min后 ,与正常对照组相比胞内钙增加 ( 73 .5± 10 .3 1) % (P <0 .0 0 1) ;在模拟缺血液中分别加入 2 0 μM、40 μM、60 μM、80 μM的Rb1灌流 10min后 ,胞内钙增加分别为 ( 19.8± 3 .41) % ,( 14 .1± 2 .78) % ,( 10 .5± 3 .62 ) %和 ( 11.5± 4.3 0 ) % ,与缺血组相比差异有显著性 (P <0 .0 1～ 0 .0 0 1)。结论 :GSRb3可通过减轻细胞内的钙超载来对缺血神经元进行保护 ;保护作用呈浓度依赖性 ,在 60Objective:To study the effect of ginsenoside Rb 3 on the intracellular free calcium in ischemic neurons. Methods:With laser scanning confocal microscope technique, we examined the effect of ginsenoside Rb 3 on cytosolic free calcium concentration in ischemic hippocampal neurons.Results:With application of ginsenoside Rb 3, we found that GSRb3 exerted inhibitory effect on free intracellular calcium concentration in ischemic hippocampal neurons. With perfusion of ischemic solution for 10 min, intracellular calcium concentration increased by (73.5±10.31) %. After a 10 min of perfusion with ischemic solution containing 20, 40, 60 and 80μM GSRb 3, intracellular calcium concentration increased only by (19.8±3 41)%, (14.1±2.78)%, (10.5±3.62)% and (11.5±4.30)%(P<0.01～0.001), respectively. The effect of 60μM GSRb3 was the most potent.Conclusions:These results suggest that ginsenoside Rb 3 can inhibit hippocampal neurons from calcium overload induced by ischemia and the effect of 60μM GSRb 3 was the most potent.

关 键 词：人参皂甙单体 脑缺血 海马神经元 细胞内游离钙 

分 类 号：R285.5[医药卫生—中药学]