小麦糖原合成酶激酶(TaGSK1)表达载体的构建及原核表达  

Construction of Triticum aestium L. Glycogen Synthase Kinase (TaGSK1) Expression Vector and Its Prokaryotic Expression

作　　者：徐涛[1] 马闻师[1] 沈银柱[1] 张庆祝[1] 齐志广[1] 黄占景[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016

出　　处：《中国农业科学》2004年第11期1593-1597,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30070471);河北省自然科学基金资助项目(301103)

摘　　要：利用PCR技术,以糖原合成酶激酶(TaGSK1)基因的pDT-23质粒为模板,对小麦Tagsk1基因进行了扩增和测序。将之转入原核表达载体pBV221,得到pBV221-gsk1;用之分别转化大场杆菌DH5α和BL21,均检测到相对分子量为43.5kD的表达产物TaGSK1蛋白,表达量分别为8%和10.8%。在0.31mol·L-1NaCl浓度下,转化子的耐盐能力比受体菌明显提高。Utilizing plasmid pDT-23 containing Tagsk1 as template, Tagsk1 was amplified and sequenced, and then ligased it into prokaryotic expression vector pBV221, then the pBV221-gsk1 was obtained. Tagsk1 expressed 43.5kD protein both in E.coli DH5α and BL21 whose expression amount were 8% and 10.8% respectively. Tagsk1 improved the transformants’ salt tolerance greatly at the concentration of 0.31 mol·L-1 NaCl.

关 键 词：小麦 糖原合成酶激酶 原核表达 耐盐性 PCR技术 

分 类 号：S512.1[农业科学—作物学]