芸薹种蔬菜杂交种及其亲本莲座期基因差异表达与杂种优势的关系  

The Relationship Between Differential Gene Expression Patterns at Rosette Stages and Heterosis in Brassica campestris and Their Hybrids

作　　者：吴才君[1] 曹家树[1] 董德坤[1] 

机构地区：[1]浙江大学蔬菜研究所

出　　处：《中国农业科学》2004年第11期1654-1659,共6页Scientia Agricultura Sinica

基　　金：教育部高等学校博士学科点专项科研基金资助项目(20030335117);浙江省重大科技资助项目(021102536)

摘　　要：以‘矮脚黄’白菜自交系(97-3-2)、‘黄心乌’自交系(003-27)、‘上海青’自交系(98-4-2)、‘黄芽菜’自交系(97-8-6)和‘白蔓菁’芜菁自交系(001-24)为亲本及其杂交所得6个F1代为材料,利用cDNA-AFLP技术,分析杂种与亲本莲座期叶片的基因差异表达类型与构成主要生物学产量性状的杂种优势关系。结果表明,基因的差异表达与杂种优势的形成有密切关系,相关分析发现双亲共沉默(ABF1)与叶片数杂种优势呈显著正相关;单亲表达沉默(UNP)与株高杂种优势呈显著正相关。With the inbred line ‘Aijiaohuang 97-3-2’, ‘Huangxinwu 003-27’, ‘Shanghaiqing 98-4-2’ of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino; syn. B. rapa L. ssp. chinensis) ,the inbred line ‘Baimanjing 001-24’ of turnip [B. campestris L. ssp. rapifera (Matzg.) Sinsk; syn. B. rapa L. ssp. rapifera], the inbred line ‘Huangyacai 97-8-6’ of Chinese cabbage-pe-tsai(B. campestris L. ssp. pekinensis Olsson; syn. B. rapa L. ssp. pekinensis) and 6 hybrids as experimental material. The relationship between differential gene expression patterns at rosette stage and heterosis of biologic yield component traits in the hybrids and their parents was analyzed by using cDNA-AFLP. Four types of differential gene expression patterns were detected between hybrids and their parents: UNF1 bands were observed only in F1 not in parents; ABF1 bands were observed in both parents but not in F1; UNP bands were observed only in one parent but not in F1 and another parent; DMP bands were observed in one parent and F1 not in another parent. Analysis results showed that ABF1 was significantly correlated with heterosis in leaf number, UNP was significantly correlated with heterosis in height of plant. These results indicated that differential expressed genes might play an important role in heterosis.

关 键 词：芸薹种蔬菜 杂交种 亲本 莲座期 基因差异表达 杂种优势 

分 类 号：S630.2[农业科学—蔬菜学]