龙须草生态型的划分及其遗传差异的AFLP分析  

Genetic Diversity and Classification of Ecotypes of Eulaliopsis binata via Morphological Traits and AFLP Markers

作　　者：姚家玲[1] 洪柳[1] 张友德[1] 胡春根[1] 骆炳山[1] 

机构地区：[1]华中农业大学,武汉430070

出　　处：《中国农业科学》2004年第11期1699-1704,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(39980016)

摘　　要：利用AFLP分子标记技术,结合形态学特征观察和叶片纤维素等成分含量的测定,将来自4个省的9个不同居群的龙须草划分为4种生态型:窄叶类型(湖北)、浓绿叶类型(广西)、红秆类型(陕西)和宽叶类型(河南);来自同一地区的不同居群之间,遗传差异很小,聚为同一种生态型;同一居群内不同无融合生殖个体之间未检测到差异;分析了不同生态型之间的遗传差异和亲缘关系;讨论了AFLP技术在无融合生殖植物的分类和遗传学研究中的应用前景。AFLP markers and morphological observation were used to identify the ecotypes of Eulaliopsis binata, which was reported as an apomictic species in Poaceae. The result showed that there is a large diversity among different ecotypes but a little within each population. By cluster analysis based on AFLP data, four ecotypes were identified as follows: slender-leaf ecotype (Hubei), deep-green-leaf ecotype(Guangxi), red-haulm ecotype (Shaanxi) and wide-leaf ecotype (Henan). The utilizations of AFLP markers in classification and further investigation of apomictic plants were discussed as well.

关 键 词：龙须草 生态型 划分 遗传差异 AFLP 无融合生殖 亲缘关系 水土保持植物 

分 类 号：Q943[生物学—植物学]