硒对苯并[a]芘诱导的小鼠肺细胞DNA损伤的作用  

Effects of selenium on benzo[a] pyrene-induced DNA damage in mouse lung cells

作　　者：余日安[1] 李晓燕[1] 鲁文清[1] 梅云华[1] 朱建林[1] 陈秀娜[1] 陈学敏[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,武汉430030

出　　处：《中华劳动卫生职业病杂志》2004年第6期445-447,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　研究亚硒酸钠对苯并 [a]芘 (BaP)诱导的小鼠肺细胞DNA损伤的影响。方法　选用雄性昆明种小鼠。亚硒酸钠采用腹腔注射处理 ,BaP灌胃染毒。BaP单独作用的剂量是 12 5、2 5 0、5 0 0mg/kg;硒和BaP联合作用组采用 0 .75、1.5 0、3.0 0mg/kg的亚硒酸钠分别与 2 5 0mg/kgBaP联合染毒。设立溶剂对照组。用单细胞凝胶电泳方法检测DNA损伤的情况。结果　 12 5、2 5 0、5 0 0mg/kgBaP组小鼠肺细胞DNA损伤程度较对照组严重 ,差异有显著性 (P <0 .0 1) ,总损伤细胞百分率分别为4 3.5 0 %、84 .0 0 %、95 .6 3% ,较对照组 (9.75 % )明显增高 ,差异亦有显著性 (P <0 .0 1) ,且存在着明显的剂量 -反应关系。 0 .75、1.5 0、3.0 0mg/kg的亚硒酸钠对 2 5 0mg/kgBaP诱导的小鼠肺细胞DNA损伤具有明显的拮抗效应 ,1.5 0mg/kg亚硒酸钠的拮抗作用优于 0 .75、3.0 0mg/kg的亚硒酸钠 ,差异有显著性 (P <0 .0 5 )。结论　 12 5～ 5 0 0mg/kg的BaP可以引起小鼠肺细胞DNA损伤 ,而 0 .75～ 3.0 0mg/kg的亚硒酸钠对 2 5 0mg/kgBaP诱导的小鼠肺细胞DNA损伤具有明显的拮抗作用。Objective To explore the effects of selenium on DNA damage induced by benzo[a]pyrene(BaP) in mouse lung cells. Methods Sodium selenite was given to Kunming male mice by i.p. and BaP was given by oral gavage.The control group was given solvent only with the same method.DNA damage was detected by single cell gel electrophoresis(or comet assay). Results The damage degrees in mice treated with 125,250 and 500 mg/kg of BaP were more severe than that of control( P <0.01).The rates of comet cells in the BaP treated groups(43.50%,84.00%,95.63%) were significantly higher than that of control(9.75%, P <0.01),and there was obvious dose response relationship.0.75,1.50 and 3.00 mg/kg of sodium selenite presented antagonistic effects against DNA damage induced by 250 mg/kg of BaP in mouse's lung cells.The antagonistic effect of sodium selenite at the dose of 1.50 mg/kg was better than those of sodium selenite at the doses of 0.75,3.00 mg/kg. WT5”H Conclusion BaP at the doses of 125～500 mg/kg could significantly induce DNA damage of lung cells in mice.0.75～3.00 mg/kg of sodium selenite could inhibit DNA damage of lung cells in mice induced by 250 mg/kg of BaP.

关 键 词：DNA损伤 小鼠 肺细胞 亚硒酸钠 鼠肺 诱导 对照组 苯并[A]芘 染毒 拮抗作用 

分 类 号：R96[医药卫生—药理学]