异氟烷预处理对离体大鼠心肌缺血再灌注损伤的影响  

Effects of isoflurane preconditioning on myocardial ischemia/reperfusion injury in isolated rat hearts

作　　者：燕宪亮[1] 曾因明[1] 许铁[2] 吕建农[2] 张育才[1] 曹汉忠[1] 于常州[1] 

机构地区：[1]江苏省麻醉医学研究所,江苏徐州市221002 [2]徐州医学院附属医院急救中心,江苏徐州市221002

出　　处：《心血管康复医学杂志》2004年第6期527-530,共4页Chinese Journal of Cardiovascular Rehabilitation Medicine

摘　　要：目的:采用Langendorff离体心脏灌注模型,研究异氟烷预处理对离体大鼠心肌缺血再灌注损伤的影响.方法:24只SD大鼠随机分为4组,每组6只,分别为缺血再灌注损伤组(IR组)、异氟烷预处理1组(IsoP 1组)、异氟烷预处理2组(IsoP 2组)和异氟烷预处理3组(IsoP 3组).监测复灌后心功能恢复情况、冠脉流出液中磷酸肌酸激酶(CK)、乳酸脱氨酶(LDH)的释放量和心肌存活面积的变化.结果:复灌期间3组IsoP心脏各对应时间点的LVEDP均显著低于对照组(P＜0.05～＜0.01);再灌注30 min时IsoP各组LVDP的恢复均高于IR组(P＜0.05),IsoP3组±dp/dtmax在再灌注30 min时的恢复百分比均高于IR组(P＜0.05),IsoP1组+dp/dt max高于IR组(P＜0.05);复灌后异氟烷预处理组各时间点的CK、LDH释放量均低于IR组(P＜0.01);IsoP2组、IsoP1组和IsoP3组心肌存活面积百分比均高于IR组(P＜0.01);预处理各组之间比较无显著性差异(P＞0.05).结论:IsoP对大鼠离体缺血再灌注心肌有保护作用,可以显著减轻心肌细胞的损伤,改善心功能,增加心肌存活面积.Objective: To study the effects of isoflurane preconditioning on myocardial ischemia reperfusion injury in isolated rat heart by Langendorff apparatus. Methods: Twenty-four isolated SD fat hearts were randomly divided into four groups: IR, IsoP 1. IsoP 2 and IsoP 3 group (6 cases in each group) after equilibration. Results: Compared with IR group, the LVEDP was much lower (P<0. 05-<0. 01), the recovery of LVDP、±dP/dtmax、 LVDP×HR was better (P<0. 05-<0. 01); the release of CK and LDH was significantly lower (P<0. 01) in IsoP groups during reperfusion. The percentages of survival areas of myocardium in IsoP groups were higher (P<0. 01). Conclusion:IsoP have myocardium protection on isolated rat ischemia reperfusion myocardium, can attenuate myocardium ischemia/ reperfusion injury, improve the cardiac function and increase the survival areas after reperfusion.

关 键 词：异氟烷 心肌缺血再灌注损伤 离体大鼠 心肌存活 IR CK 预处理 心功能恢复 对照组 冠脉流出液 

分 类 号：R542[医药卫生—心血管疾病] R614[医药卫生—内科学]