胸腺五肽一级结构的电喷雾质谱分析  

Determination of the first-order structure of thymopentin(TP5)by elec trospray ionization mass spectrometry

作　　者：周红华[1] 马仁玲[1] 黄海燕 濮社班[3] 盛龙生[1] 

机构地区：[1]中国药科大学分析测试中心,江苏南京210009 [2]江苏长澳医药有限公司,江苏南京210007 [3]中国药科大学中药学院,江苏南京210038

出　　处：《西北药学杂志》2004年第6期243-244,共2页Northwest Pharmaceutical Journal

摘　　要：目的测定胸腺五肽的相对分子质量及其一级结构。方法应用电喷雾质谱法(ESIMS)及源内碰撞诱导解离技术对胸腺五肽进行测定,根据其典型碎片离子确证其氨基酸序列及相对分子质量。结果测得胸腺五肽准分子离子峰(M+H)+m/z680.3及(M+Na)+m/z702.2,(M+2Na-H)+m/z724.3及(M+3Na-2H)+m/z746.2,表明胸腺五肽的相对分子质量为679.4,与理论值一致。结论胸腺五肽的相对分子质量及一级结构与理论值相符。Objective To determine the molecular weight and the first-order structure of thymopentin. Methods The molecular weight and amino acid sequence of thymopentin were determined by electrospray ionization mass spectrametry. A series of typical fragment ions were obtained by in-source collision-induced dissociation (CID). The b series of fragment ions were obtained by adjusting the fragmentor voltage. Results The quasi-molecular ions were 680. 3(M + H)+and 702. 2(M+Na)+, 724. 3(M + 2Na-H)+, 746. 2(M + 3Na-2H) + . Conclusion The molecular weight and first-order structure of thymopentin were confirmed.

关 键 词：胸腺五肽 相对分子质量 一级结构 电喷雾质谱 

分 类 号：R927.2[医药卫生—药学]