高效液相色谱法测定黄连上清胶囊中大黄素及大黄酚的含量  

Determination of Emodin and Chrysophanol in Huanglianshangqing Capsule by HPLC

作　　者：樊和平[1] 

机构地区：[1]安徽省药品检验所,安徽合肥230061

出　　处：《现代中药研究与实践》2004年第6期42-43,共2页Research and Practice on Chinese Medicines

摘　　要：目的　建立黄连上清胶囊中大黄素及大黄酚含量的高效液相色谱测定方法。方法　采用 C1 8柱 ,甲醇 - 0 .2 %磷酸 (80∶ 2 0 )为流动相 ,流速 :1.0 m L/ min。检测波长 :2 5 4 nm。结果　平均回收率大黄素为 98.4 %(n=5 ) ,RSD=1.1% ;大黄酚为 98.5 % (n=5 ) ,RSD=0 .9%。结论　试验表明 ,方法简便、快速、结果准确 。Objective To develop an HPLC assay for determining Emodin and Chrysophanol in Huanglianshangqing Capsule.Method A C 18 column was used. The mobile phase was with a mixture of methanol-0.2% Phosphoric Acid(80∶20).The flow rate was 1.0ml/min,and wavelength was 254 nm.Result The average recovery rates of Emodin and Chrysophanol were 98.4%(n=5)and 98.5%(n=5) respectively; RSD were 1.1% and 0.9% respectively. Conclusion The method is proved simple, accurate, highly sensitive and recommendable.

关 键 词：大黄素 大黄酚 胶囊 黄连 高效液相色谱法 酚含量 检测波长 C18柱 平均回收率 试验 

分 类 号：R286.0[医药卫生—中药学] R927.2[医药卫生—中医学]