烟碱-磷钼杂多酸缔合微粒体系的光谱研究及分析应用  

Spectral Properties of Nicotine-PMo Heteropoly Acid andIts Analytical Application

作　　者：梁爱惠[1] 蒋治良[1] 李振中[1] 

机构地区：[1]广西师范大学资源与环境学系,广西桂林541004

出　　处：《分析测试技术与仪器》2004年第4期218-221,共4页Analysis and Testing Technology and Instruments

基　　金：广西自然科学基金(014403);广西师范大学博士科研基金资助项目.

摘　　要：采用分光光度法和共振散射光谱法研究了小烟碱 磷钼杂多酸缔合微粒体系.在波长300～800nm范围内,波长愈短缔合微粒体系的吸光度值愈大,在400nm处的吸光度与烟碱浓度在0.50～50.0μg/mL范围成良好线性关系,检出限(3σ)为0.1μg/mL.据此建立了一个测定烟草中烟碱含量的分光光度法.该法灵敏、简便、快速、准确、重复性好,平均回收率为99%～103%,相对标准偏差RSD为0.9%～1.3%.共振散射光谱研究结果表明,该体系存在烟碱-磷钼杂多酸缔合微粒,该微粒在420nm处存在共振散射效应.The association particle systems of nicotine (NT)\|PMo heteropoly acid were studied spectrophotometrically and resonance scattering spectroscopitrically. The absorbance values decreases with the wavelength in the range of 300-800 nm. The A_(400 nm) is linear to the nicotine concentration in the range of 0.16～24 μg/mL. Its detection limit is 0.08 μg/mL NT. A new spectrophotometric method has been proposed for the determination of NT in real samples, with some advantages including high sensitivity, simplicity, rapidity and accuracy. The means recovery is 100.5%. The RSD is 1.00%(n=5). The results of resonance scattering spectra show that there are (NT-PMo)n association particles which exhibit resonance scattering effect at 420 nm.

关 键 词：烟碱 磷钼杂多酸 缔合微粒 分光光度法 

分 类 号：O657.33[理学—分析化学]