高胆固醇血症对鹌鹑在体动脉内皮细胞损伤的定量研究  

THE QUANTITATIVE ANALYSIS ON THE AORTIC ENDOTHELIAL INJURY OF QUAILS WITH HYPERCHOLESTEROLEMIA IN VIVE

作　　者：胡维诚[1] 徐明义[1] 刘玉梅[1] 李莉[1] 周庚寅[2] 李锦红[1] 王韶山[1] 邵念芳[3] 

机构地区：[1]山东医科大学病理生理学教研室 [2]山东医科大学病理解剖学教研室 [3]山东中医学院附属医院

出　　处：《山东医科大学学报》1993年第3期184-188,共5页Acta Academiae Medicinae Shandong

基　　金：国家自然科学基金(3880395)

摘　　要：为定量分析高胆固醇血症对动脉内皮细胞的损伤,并观察调脂通脉灵对此损伤可能存在的保护作用,采用内皮细胞铺片法和放射自显影技术,对鹌鹑在体动脉内皮细胞再生进行了定量分析。研究发现,高胆固醇组动脉内皮细胞排列不规则、密度增加,~3H-TdR标记率为2.05±4.85‰,与对照组相比(~3H-TdR标记率为0.81±0.87‰)差异有显著性。经调脂通脉灵治疗后,血脂浓度明显降低,~3H-TdR标记率随之下降。提示:通过对内皮细胞再生的定量分析,可对内皮细胞的损伤程度作出定量分析。The aortic endothelial cell replication quails with hypercholes- terolemia was quantitatively researched by special method of en-face prepa- ration and ~3H-thymidine autoradiography.The endothelial cells arrange disorderly with high density.The labelling rate of ~3H-TdR is 0.205±0.485% per 24 hours in hypercholesterolemia group and significantly higher than that(0.081±0.087% per 24 hours)in control group(P<0.01).The cholesterol concentration was reduced by the treatment of TZTML(traditional Chinese medicine)and the labelling rate was reduced too.The results indi- cate that the endothelium injury can be quantitatively analyzed with the index of ~3H-TdR labelling rate.

关 键 词：动脉 内皮细胞 损伤 高胆固醇血症 

分 类 号：R543.5[医药卫生—心血管疾病]