胺化超高交联吸附树脂的制备及其对喜树碱和喜果苷的色谱分离  

Preparation of Aminated Macroporous Hypercrosslinked Resins and Their Application in Chromatographic Separation of Camptothecin and Vincoside Lactam

作　　者：王瑞芳[1,2] 史作清[1] 施荣富[1] 张静泽[1] 杨文泓[1] 吴伟[1] 

机构地区：[1]南开大学吸附分离功能高分子材料国家重点实验室,高分子化学研究所,天津300071 [2]三达膜科技(厦门)有限公司

出　　处：《应用化学》2005年第1期24-29,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20174017)资助项目

摘　　要：研究了所合成的大孔超高交联吸附树脂(Rf18)及其甲胺化(Rs3)、N-甲基乙酰胺化(Rs4)、二甲胺化(Rs5)、三甲胺化(Rs6)后对喜树碱和喜果苷的吸附性能及柱层析分离效果. 结果表明,超高交联吸附树脂胺化后,对喜树碱和喜果苷的吸附量及吸附选择性显著增大,如季铵盐树脂(Rs6)对2种生物碱的分离因子高达114.Rf18、Rs3、Rs5树脂可经分步洗脱,使喜树碱和喜果苷完全分离;Rs6树脂吸附后,喜树碱用有机溶剂难以洗脱,但用质量分数为80%的酸性(pH＝4)乙醇水溶液淋洗时,喜树碱很快被洗脱.经层析树脂柱分离,与上柱前相比喜树碱的质量分数可提高3～6倍,喜果苷提高2～3倍.Macroporous hypercrosslinked resin(Rf18) was synthesized from styrene and decorated by loading methyiamine(Rs3), N methylacetamido(Rs4), dimethylamine(Rs5) and trimethylamine(Rs6) respectively . The adsorption and chromatographic separation of camptothecin and Vincoside Lactam(VCS LT) by the synthesized resins were compared. Their adsorption capacity and adsorption selectivity for camptothecin and vincoside lactam were found to be remarkably increased. For quaternary ammonium resin(Rs6), the separation impact( α c/v ) was 114. Camptothecin and vincoside lactam could be effectively separated with resins Rf18 (Rs3, Rs5). But camptothecin could not be washed out from Rs6 with organic solvent, it could be easily washed out with acidic solution of 80% ethanol water(pH=4).

关 键 词：喜树碱 喜果苷 胺化超高交联吸附树脂 吸附性能 层析分离 

分 类 号：O647.3[理学—物理化学]