人重组干扰素-α及白细胞介素-2对病理性瘢痕成纤维细胞增殖的影响  

Mechanism of the effects of rhIFN-α and rhIL-2 on the proliferation of hypertrophic scar fibroblasts

作　　者：吴勇[1] 刘流[1] 彭庆芳[1] 

机构地区：[1]昆明医学院第一附属医院口腔颌面外科,650032

出　　处：《中华实验外科杂志》2005年第1期41-42,共2页Chinese Journal of Experimental Surgery

基　　金：云南省应用基础研究基金资助项目 (2 0 0 0C0 0 61 )

摘　　要：目的　观察人重组干扰素- α(rhIFN-α)及白细胞介素-2 (rhIL-2 )对病理性瘢痕成纤维细胞生物学行为的影响 ,探讨细胞因子对病理性瘢痕的作用机制。方法　对 5例临床病理性瘢痕标本进行成纤维细胞体外培养 ,采用流式细胞术方法进行观察、分析 ,以研究细胞因子rhIFN-α及rhIL-2 (浓度分别为 2 5 0 ,5 0 0 ,75 0U/ml ,每组数n =6)对病理性瘢痕成纤维细胞增殖、细胞周期、Fas、Bcl-2表达量的影响。结果　加入rhIFN-α 72h后检测 ,S-G2-M期细胞百分数降低 (P <0 .0 5 ) ,Fas、Bcl-2蛋白表达量 ,分别显示升高 (P <0 .0 5 )和降低 (P <0 .0 5 ) ;加入rhIL-2后S-G2-M期细胞百分数明显升高 (P <0 .0 1) ,Fas、Bcl-2蛋白表达量 ,两者均有升高 (P <0 .0 5 ,P <0 .0 1)。结论　rhIFN-α是成纤维细胞负性调节因子 ,对成纤维细胞的生长趋势及增殖活性有明显抑制作用。rhIL-2作为成纤维细胞正性调节因子 ,促进成纤维细胞的生长增殖。Objective To investigate the effects of rhIFN α and rhIL 2 on on the biological behaviors of hypertrophic scar fibroblasts and the mechanism of cytokines on the hypertrophic scar.Methods The fibroblasts derived from 5 cases of hypertrophic scar cultivated in vitro were observed and analyzed by using flow cytometry (FCM).The effects of rhIFN αor rhIL 2 (250 U/ml,500 U/ml,750 U/ml, n =6 in each group) on biological behaviors (cell proliferaiton,cell cycle,expression of Fas and Bcl 2) of hypertrophic scar fibroblasts were studied.Results Seventy two h after the addition of rhIFN a,the percentage of the S G2 M phase cells was signficantly decreased ( P < 0.05), the expression of Fas and Bcl 2 proteins was increased and decreased respectively (both P < 0.05). After addition of rhIL 2,the percentage of the S G2 M phase cells was obviously elevated ( P < 0.01) and the expression of both Bcl 2 ( P < 0.01) and Fas ( P < 0.05) was increased.Conclusion As a negative modutory factor,rhIFN αcan suppress growth and proliferation of hypertrophic scar fibroblasts;As a positive modutory factor,rhIL 2 can promote the growth and proliferation of hypertrophic scar fibroblasts.

关 键 词：成纤维细胞增殖 病理性瘢痕 RHIL-2 IFN-α 重组干扰素 白细胞介素-2 升高 标本 生长 体外培养 

分 类 号：R622[医药卫生—整形外科]