兔在体肺保存模型的建立及其意义  

Establishment of rabbit in situ pung preservation model and its significance

作　　者：付庆林[1] 齐战[1] 张风伟[1] 陈忠堂[1] 邵东[2] 吕立红[2] 尹金岭[2] 

机构地区：[1]山东大学医学院外科学系,济南250012 [2]山东大学医学院手术学研究室,济南250012

出　　处：《中华实验外科杂志》2005年第1期106-107,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　建立一种能筛选和鉴定肺保护液保护效果的兔在体肺保存模型。方法　新西兰白兔 40只 ,经左侧开胸 ,掀开胸壁 ,游离左上肺动脉 ,穿刺置管 ,切除左肺上叶 ,经左心房置管入左肺下静脉。阻断左肺根后 ,自肺动脉灌注肺保护液 ,经肺静脉管引流 ,完毕后将左肺下叶放入特制的肺保存器内低温冷藏 2h ,移去肺保存器 ,开放肺门再灌注 3h。在实验过程中取标本观察肺保存效果。结果　在预实验阶段 ,10只动物因出血死亡 ,或因出血导致的后续操作无法进行而致失败 5只 ,成功率 5 0 %。经过改进后在正式实验阶段 ,3 0只动物模型均制作成功 ,术中血压、心率及体温等各项生理指标稳定 ,与开胸前相比 ,无统计学意义差异 (P <0 .0 5 ) ,动物对手术的耐受性好。结论　兔在体肺保存模型制作简单 ,过程符合生理 ,模型稳定、安全、实用 ,是研究肺灌注液保存效果的理想的急性和慢性实验动物模型。Objective To establish a rabbit in situ lung preservation model for the study on the effectiveness of lung slush solution.Methods Forty New Zealand rabbits were selected as experimental subjects.By left thoracotomy,left superior pulmonary artery was separated and was inserted into a catheter and then left upper lobe was removed.Another catheter was inserted into the left inferior pulmonary vein via left artrial wall.Both catheters were connected to sensors to detect the pressure.After left lung root was clamped,lung flush solution was perfused through the catheter in the left superior pulmonary artery and was drained via the catheter in the left inferior pulmonary vein.The left lower lung lobe was put and stored in a specially made lung container for 2 h.Thereafter,the lung root was declamped for 3 h.After samples were collected,the subjects were executed by euthanasia.Results In the pre experiment stage,5 rabbits out of 10 failed to finish the model establishment due to hemorrhage,with the successful rate of 50%.With the improved technique,at the formal experimental stage,all the 30 rabbit models were set up successfully,and the physiological parameters including blood pressure,heart rate and body temperature during operation were all stable compared with pre operation ( P > 0.05). Conclusion Rabbit in situ lung preservation model,as an ideal model to investigate the effect of lung slush solution,is simple,physiological,stable,safe and practical.

关 键 词：开胸 肺保护液 出血 保存 置管 肺门 左心房 动物 标本 生理 

分 类 号：R655.3[医药卫生—外科学]