调心方活性部位对大鼠海马脑片CA1区诱发LTP的影响  

Effect of Active Fraction of Tiaoxin Recipe on Long term Potentiation of CA1 in Rat Hippocampal Slices

作　　者：乔海法[1] 杨胜[1] 周文霞[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国实验方剂学杂志》2005年第1期31-34,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金重点项目 (39830 4 50 )

摘　　要：目的 :研究调心方活性部位A对大鼠海马脑片诱发长时程增强 (LTP)效应的影响。方法 :采用细胞外微电极记录技术 ,记录大鼠海马脑片CA1区诱发群峰电位 (PS) ,然后施以 10 0Hz,10 0串的强直刺激诱发LTP。结果 :调心方活性部位A对大鼠海马脑片正常PS的形状、幅度无影响 ,提示并不影响海马的基础突触传递。给予强直刺激后 ,高浓度活性部位A可以明显增大LTP幅度 ,提示具有促进和维持LTP的作用。结论 :调心方活性部位A可能是调心方促进和维持大鼠海马脑片诱发LTP的有效成分之一 ,对LTP的促进作用也是调心方活性部位A益智作用的机制之一。Aim:To investigate the active fraction A(TRX A) of Tiaoxin Recipe(TXR) on long term potentiation(LTP) of CA1 area in the rat hippocampl slices.Methods:LTP in CA1 area of rat hippocampal slices,which was induced by high frequency stimulation(100Hz,100pulses),was recorded with extracellular recording technique.Results:TXR A or TXR showed no effect on baseline synaptic responses.Compared with the slices pre incubated and perfused with decoction free artificial cerebrospinal fluid(ACSF),population spike(PS) amplitude of slices exposed to TXR A or TXR ACSF was significantly increased after high frequency stimulation.Conclusion:TXR A is one of TXR active fractions which facilitate LTP in CA1 area of slices from rat.

关 键 词：调心方 长时程增强 海马脑片 

分 类 号：R285.5[医药卫生—中药学]