干旱胁迫下刚毛柽柳消减文库的构建及分析  

Construction and analysis of Tamarix hispida suppression subtractive hybridization library under drought stress

作　　者：刘桂丰[1,2] 侯英杰[3] 王玉成[3] 褚延广[3] 

机构地区：[1]北京林业大学生物科学与技术学院 [2]东北林业大学林学院,哈尔滨150040 [3]东北林业大学林学院

出　　处：《植物研究》2005年第1期69-73,共5页Bulletin of Botanical Research

基　　金：国家 973计划资助项目 (G19990 160 0 0 )

摘　　要：以干旱胁迫下的刚毛柽柳根部组织cDNA为tester,正常生长的刚毛柽柳根部组织cDNA为driver,利用抑制性消减杂交技术 (SSH)构建了干旱胁迫下刚毛柽柳根部组织的消减文库。文库克隆的重组率为 95 % ,插入片段大部分集中在 2 5 0～ 60 0bp之间。通过对文库阳性克隆的随机测序 ,获得了如Mn SOD、myb相关蛋白、锌指蛋白等 1 7种与干旱胁迫相关的基因 ,它们涉及了植物的渗透调节、信号传递、转录调控、活性氧清除等方面。所得EST序列已被GenBank收录。Using cDNA from Tamarix hispida roots treated with drought stress as tester and cDNA from Tamarix hispida roots in normal growth as driver, suppression subtractive hybridization (SSH) was employed to construct cDNA subtracted library. In the library, the rate of recombination was 95%, the size of inserts was 250～ 600 bp . 17 drought stress associated genes were obtained by DNA sequencing the positive clones picked randomly,such as Mn SOD myb related protein zinc finger protein, etc. These genes included osmotic regulator signal component regulatory protein and antioxidant enzyme. GenBank had accepted all the sequences. The research had established a basis for cloning stress resistance genes and further studying genes expression in Tamarix hispida roots under drought stress.

关 键 词：干旱胁迫 刚毛柽柳 消减文库 抑制性消减杂交技术 表达序列标签 抗旱性 抗旱基因 

分 类 号：S793.5[农业科学—林木遗传育种]