人乳头瘤病毒16型DNA转化人胚肺细胞的实验研究  

STUDIES ON TRANSFORMATION OF HUMAN EMBRYO LUNG CELLS BY HUMAN PAPILLOMAVIRUS TYPE 16 DNA

作　　者：高泓[1] 于修平[1] 董杰德 曹宗离 仇素英 

机构地区：[1]微生物学教研室

出　　处：《山东医科大学学报》1993年第1期23-26,共4页Acta Academiae Medicinae Shandong

摘　　要：人乳头瘤病毒(HPV)的16,18,31,33型等与宫颈癌的发病有关,其中HPV16与宫颈癌关系密切。为进一步研究HPV16的致癌性,我们用克隆的HPV16 DNA(2μg/10~5细胞)转染体外培养的人胚肺细胞,并进行了细胞存活时间、血清依赖性、着壁依赖性、间接免疫酶检测、HPV16 DNA、同源序列检测、染色体核型等生物学的研究。结果表明,转染细胞存活时间延长、在软琼脂培养基中形成集落、HPV16特异抗原得以表达、HPV16 DNA的同源序列存在于细胞中。表明本实验用HPV16DNA转染的人胚肺细胞具备转化细胞的某些特征,HPV16有使人胚肺细胞转化的作用。Some of human papillomavirus(HPV)types,such as type 16, 18,31,33 etc.,have been implicated in the etiological role of human cervical carcinoma.Among these types,HPV 16 is most commonly detected in biopsy specimens of the cancer.In order to demonstrate further the oncogenic potential of HPV 16,we studied the in vitro transformation of HPV 16 DNA on human embryo lung cells(HEL).HEL cell monolayers were transfected with cloned HPV 16 DNA(2μg/10~5 cells) by the DNA-calcium phosphate precipitation method.The following biological properties of the transfected cells were examined:1.life span;2.serum dependency;3.colony formation in soft agar medium;4.chromosome type;5.expression of HPV 16 antigen; 6.existence of HPV 16 DNA homogenic sequences. Results revealed:1.life span of the cells extended;2.colonies formed in soft agar medium;3.HPV 16 specific antigen was expressed;4.HPV 16 DNA homogenic sequences existed in the cells. All the above results showed that the cells possesed the characteristics of transformed cells.This study demonstrated that HEL cells can be transformed by HPV 16 DNA.

关 键 词：乳头瘤病毒 脱氧核糖核酸 细胞转化 

分 类 号：R373.9[医药卫生—病原生物学]