柱前衍生化HPLC法测定百多宁注射液中的脂肪酸含量  

Detection of fatty acid in the Baiduoning Injection by the pre-column derivation HPLC

作　　者：丁怡[1] 唐星[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《沈阳药科大学学报》2005年第1期29-32,共4页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省重大课题项目 (2 0 0 2 2 2 6 0 0 5 )

摘　　要：目的建立以HPLC法测定百多宁注射液中脂肪酸含量的方法。方法采用无水硫酸钠破乳方法分离百多宁注射液中的油相。以 2 ,4′ 二溴苯乙酮为衍生化试剂 ,18-冠 - 6醚为相转移催化剂 ,采用C8反相柱 ,以乙腈 -水 (V∶V =75∶2 5 )为流动相等度洗脱 ,正十七烷酸为内标 ,一次基线分离 4种脂肪酸。结果亚油酸在 4 6～ 30 7ng内、软脂酸在 2 7 2～ 181 0ng内、油酸在 14 4～96 0ng 内、硬脂酸在 9 7～ 6 5 1ng内质量与相对峰面积线性关系良好 ,相关系数分别为 0 9997、0 9999、0 9995、0 9996。平均回收率分别为 98 2 %、97 5 %、99 8%、10 2 3% ,RSD分别为2 6 %、3 0 %、1 2 %、2 4 %。结论方法重现性好、定量准确 。Objective To develop a method for the determination of fatty acid in the Baiduoning Injection by Pre-column HPLC.Method The oil phase in the Baiduoning Injection was separated by adding anhydrous Na 2SO 4,then the oil phase was extracted with petroleum and acetone.Fatty acid was derivatized with p-bromophenacylbromide as derivative and 18-crown-6 as catalyst.The method used C 8 column material and isocratic acetonitrile-water eluent and the internal standard was heptadecanoic acid.Results The standard curves of linoleic,palmitic,oleic,steraric acid were linear within the range of 46～307 ng,27.2～181 ng,144～960 ng,9.7～65.1 ng and the coefficient were 0.999 7, 0.999 9, 0.999 5, 0.999 6. The four fatty acid recoveries were 98.2%,97.5%,99.8%,102.3% , and the RSD were 2.6%,3.0%,1.2%,2.4% individually.Conclusion The present method is reliable and relatively simple for the determination of fatty acid in the Baiduoning Injection.

关 键 词：含量测定 注射乳剂 脂肪酸的测定 柱前衍生化 高效液相色谱法 

分 类 号：R944.9[医药卫生—药剂学]