人参皂苷Re对MPTP致帕金森病模型小鼠多巴胺能神经元的保护作用  

Protective effects of ginsenoside Re on the dopaminergic neurons in the MPTP-induced mouse model of PD

作　　者：徐琲琲[1] 刘纯青[2] 马涛[1] 周晓棉[1] 曹颖林[1] 张万琴[2] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]大连医科大学基础学院,辽宁大连116027

出　　处：《沈阳药科大学学报》2005年第1期36-44,共9页Journal of Shenyang Pharmaceutical University

摘　　要：目的研究人参皂苷Re对 1 甲基 4 苯基 1,2 ,3,6 四氢吡啶 (1 methy 4 phenyl 1,2 ,3,6 te trahydropyridine ,MPTP)诱致帕金森病模型小鼠多巴胺能神经元的保护作用及其可能机制。方法通过给C5 7BL小鼠皮下注射MPTP制备帕金森病 (parkinson’sdisease ,PD)模型 ,灌胃给予人参皂苷Re预处理后 ,运用逆转录 聚合酶链式反应 (RT PCR)、免疫组织化学染色以及图像分析等技术分别对纹状体前脑啡肽原 (preproenkephalin ,PPE)mRNA、前强啡肽原 (preprodynorphin ,PPD)mRNA的表达水平和黑质酪氨酸羟化酶 (TH)、γ 氨基丁酸 (GABA)免疫反应阳性神经元数目等多项指标进行考察。结果Re能使黑质致密部 (SNc)的TH阳性神经元和黑质网状部 (SNr)的GABA阳性神经元数目显著增多 ;Re高剂量预防组 (2 6mg·kg-1)PPD扩增产物较模型组显著增加 (P <0 0 5 ) ;Re预防组的PPE扩增产物与模型组比没有显著差异。结论人参皂苷Re对MPTP诱致帕金森病小鼠黑质多巴胺能神经元具有明显的保护作用 ,其作用可能与改变GABA能神经元以及PPDmRNA表达水平 ,从而调节PD中直接回路和间接回路的兴奋Objective To investigate the protective effect of ginsenoside Re on dopaminergic neurons and its mechanisms in 1-methy-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced mouse's model of the Parkinson's disease (PD).Method C57BL mice were administrated i.s.c with MPTP to establish PD model.The pretreatment groups were given different doses of ginsenoside Re (6.5,13, 26 mg·kg -1) i.g.for 13 days.Tyrosine hydroxylase (TH) and γ-aminobutyric acid(GABA) expressions were investigated by the immunohistochemistry (ICC) method.RT-PCR was used to detect the expression of PPD mRNA and PPE mRNA.Result The pretreatment with ginsenoside Re (13, 26 mg·kg -1) is shown to markedly increase TH-positive neurons in the SNc and GABA-positive neurons in SNr.Simultaneously,Re is found to up-regulate expression of PPD mRNA (P<0.05) compared with the model group.Conclusion Ginsenoside Re shows protective effects on dopaminergic neurons in the MPTP-induced mouse's model of PD.It may relate to changing the expression of GABAergic neurons and PPD mRNA and regulating the balance of excitation and inhibition between the direct circuit and indirect circuit by Re.

关 键 词：人参皂苷RE 1-甲基-4-苯基-1 2 3 6-四氢吡啶 前脑啡肽原 前强啡肽原 γ-氨基丁酸 酪氨酸羟化酶 

分 类 号：R94[医药卫生—药剂学]