八种稀土金属配合物和铂配合物抗HIV活性的研究  

The anti-HIV-1 activity of 8 rare earth complexes and platinum complex

作　　者：梁兆玲[1] 曾涛[2] 汪旭[1] 贲昆龙[2] 

机构地区：[1]云南师范大学生命科学学院,云南昆明650092 [2]中国科学院昆明动物研究所,云南昆明650223

出　　处：《云南师范大学学报（自然科学版）》2005年第1期40-43,共4页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：云南省自然科学基金资助(2001C0054M)

摘　　要：　为了寻找治疗艾滋病的新药,研究了8种稀土金属(镧、铒、镝、钆、镨、镱、钐、钕)配合物和铂配合物的抗HIV活性。我们以C8166细胞系为指示细胞,测定样品对HIV-1IIIB诱导的细胞合胞体形成的抑制作用,并以MTT方法测定它们对细胞存活率的影响,以研究稀土和铂配合物对细胞的毒性。根据公式,计算出其CC50和EC50值,并进一步计算出其TI值。结果表明,镧配合物、钐配合物和铂配合物的EC50值分别为0.006μg/ml、74.28μg/ml和21.54μg/ml,它们的治疗指数分别为63、21和45,具有一定的抗HIV-1活性。其它的配合物具有很低或者不具有抗HIV-1活性。To find a novel anti-HIV/AIDS drug, we detected the anti-HIV-1 activity of rare earth lanthanum, erbium, dysprosium, gadolinium, praseodymium, ytterbium, samarium, neodymium complexes, and platinum complex. The anti-HIV activity and cytotoxicity of these complexes were determined by the inhibitory assay of HIV-1 induced syncytial formation and MTT colorimetric assay, respectively. For these assay, a T cell adapted HIV-1IIIB viral strain and C8166 cells were used. The CC_(50) (50% cytotoxic concentration)、 EC_(50) (50% effective concentration) and TI (therapeutic index) of the above compounds were further calculated. The results showed that the EC_(50) of the lanthanum and samarium complexes and platinum complex were 0.006μg/ml, 74.28μg/ml and 21.54μg/ml, respectively; And their therapeutic indexes were 63, 21 and 45,respectively. The other rare earth complexes showed very low therapeutic index or no anti-HIV activity.

关 键 词：抗HIV活性 铂配合物 抗HIV-1 治疗指数 细胞 艾滋病 新药 镧配合物 稀土金属 公式 

分 类 号：N941[自然科学总论—系统科学] R512.91[医药卫生—内科学]