新城疫病毒HeB02分离株F基因的序列分析  

Nucleotide Sequence Analysis and Expression of the F Gene of NDV Isolate HeB02

作　　者：孙一敏[1] 边艳青[1] 柏佳宁[1] 孙继国[2] 赵宝华[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]河北农业大学动物科技学院,河北保定071001

出　　处：《中国兽医学报》2005年第1期4-6,共3页Chinese Journal of Veterinary Science

基　　金：河北省重点学科基金资助项目 (2 0 0 10 2 )

摘　　要：根据国外已发表的新城疫病毒 F基因序列 ,设计了 1对引物 ,并以 RT- PCR特异性扩增出新城疫病毒 He B0 2分离株的 F基因 ,基因产物大小为 1.6 3kb,与设计相符。对其进行序列测定后 ,与其他标准毒株 F4 8E9、L a Sota和Clone30的 F基因进行了同源性比较 ,结果表明 ,He B0 2株与国内标准强毒株 F4 8E9及目前广泛应用的弱毒疫苗株L a Sota和 Clone30的 F基因核苷酸序列的同源性分别为 88.1%、84 .9%和 83.8%。由此可以看出 ,He B0 2分离株与标准毒株和疫苗株相比 ,在One pair of primers for amplifying the F gene of NDV were designed, with which the predicted 1.63 kb fragment had been amplified via RT-PCR from NDV strain HeB02. The F gene of strain HeB02 was sequenced and compared with standard strains such as F_(48)E_9, LaSota and Clone30. The results showed that the homologies of nucleic acids between HeB02 strain and F_(48)E_9, LaSota and Clone30 strains were 88.1%, 84.9% and 83.8%, respectively. The difference of the F gene of NDV strain HeB02 with those of other NDV standard strains had been demonstrated.

关 键 词：F基因 新城疫病毒 分离株 强毒株 弱毒疫苗株 序列分析 引物 基因产物 RT-PCR 扩增 

分 类 号：R512[医药卫生—内科学] S852[医药卫生—临床医学]