羊伪结核棒状杆菌的分离鉴定及ELISA检测方法的建立  

Isolation,Identification of Corynebacterium pseudotuberculosis and Establishment of ELISA Detection Method

作　　者：王韡[1] 张彦明[1] 邢福珊[1] 郑增忍[1] 张为民[1] 郭抗抗[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国兽医学报》2005年第1期34-36,共3页Chinese Journal of Veterinary Science

基　　金：农业行业标准制定和修订计划专项项目 (0 3 14 5 )

摘　　要：从疑似山羊干酪性淋巴结炎羊的肩前淋巴结脓肿内分离到 2株菌 ,经鉴定均为羊伪结核棒状杆菌。利用羊伪结核棒状杆菌标准菌株 (ATCC194 10株 )制成外毒素作为检测抗原 ,通过酶标抗体、阳性血清、外毒素抗原最适浓度的选择试验 ,确定适当的抗原、抗体和酶标抗体稀释浓度 ,建立了间接 EL ISA方法用于检测抗体。用建立的 EL ISA方法检测 10 0份待检羊血清 ,其中阳性血清 4份 ,阳性检出率为 4 %。Two bacteria were isolated from goats infected with caseous lymphadenitis(CLA).The isolated bacteria were identified to be Corynebacterium pseudotuberculosis.Through Corynebaterium pseudotuberlosis ATCC19410 an exotoxin was prepared as the antigen.Selecting appropriate consistency of antigen,antibody and enzyme-labelled dilute concentration,we established ELISA for examine sera antibody.The ELISA detection method was used to examine 100 samples,resulting in 4 positive with the positive percent of 4%.

关 键 词：伪结核棒状杆菌 阳性血清 分离鉴定 干酪性淋巴结炎 间接ELISA ELISA检测 山羊 抗原 外毒素 酶标抗体 

分 类 号：R446[医药卫生—诊断学] S858[医药卫生—临床医学]