高效液相色谱荧光检测法测定人血浆中麦考酚酸的浓度  

Determination of mycophenolic acid concentration in human plasma by high performance liquid chromatography with fluorescence detection

作　　者：沈杰[1] 焦正[1] 郁韵秋[2] 迟丹怡[1] 张明[3] 钟明康[1] 

机构地区：[1]复旦大学附属华山医院临床药学研究室,上海200040 [2]复旦大学药学院药物分析教研室,上海200032 [3]复旦大学附属华山医院肾内科,上海200040

出　　处：《中国新药与临床杂志》2005年第1期11-13,共3页Chinese Journal of New Drugs and Clinical Remedies

基　　金：上海市自然科学基金项目(032R14010)

摘　　要：目的:建立高效液相色谱(HPLC)荧光检测法测定人血浆中麦考酚酸浓度的方法。方法:以萘普生为内标,血浆用甲醇蛋白沉淀后直接进样。采用KromasilC8(150mm×4.6mm,5μm),柱温:25°C;流动相∶乙腈:0.04mol·L-1甘氨酸缓冲液,pH9.2(20∶80);流速:1.0mL·min-1,荧光激发波长342nm,发射波长425nm。结果:血浆内源性杂质和常用合并用药对样品测定无干扰,麦考酚酸在0.05～40mg·L-1范围内线性关系良好(r=0.9999)。麦考酚酸的日内、日间RSD均小于7%,样品3次冻融及提取后在24h内稳定性良好。结论:HPLC法快速、灵敏、准确,可用于麦考酚酸的体内分析及常规的治疗药物监测。AIM: To determine the concentration of mycophenolic acid in human plasma by HPLC with fluorescence detection. METHODS: Using naproxen as internal standard, mycophenolic acid in plasma samples was determined by HPLC with protein precipitation. HPLC analytical column was created by Kromasil C 8 (150 mm ×4.6 mm, 5 μm). Separation was carried out at 25 °C. The mobile phase consisted of a mixture acetonitrile∶0.04 mol·L -1 glycine buffer, pH 9.2 ( 20∶80) pumped at a flow rate of 1.0 mL·min -1. A fluorescence detector was set at λ ex 342 nm, λ em 425 nm. RESULTS: The intrinsic impurity and the combination used drugs in plasma did not interfere with the determination of the sample tests. There was good linear relationships(1/C weighted) between peak area ratio of mycophenolic acid to internal standard and C(r=0.999 9) within the range of 0.05- 40 mg·L -1. The precision of within-run and between-run was good. CONCLUTION: The method established in this paper can be used to determine the concentrations of mycophenolic acid in human plasma. Method can also be applied to the clinical pharmacy research in vivo.

关 键 词：免疫抑制剂 麦考酚酸 色谱法 高压液 相 荧光检测 

分 类 号：R979.5[医药卫生—药品] R927.2[医药卫生—药学]