Exendin-4对非糖尿病小鼠的血糖和糖耐量的影响  

Effects of exendin-4 on glycemie control and glucose tolerance in non-diabetic mice

作　　者：朱敏[1] 史虹莉[2] 方京冲[2] 李智[2] 胡敏[2] 黄葵香[2] 

机构地区：[1]复旦大学附属华山医院老年科,上海200040 [2]复旦大学附属华山医院内分泌科,上海200040

出　　处：《中国新药与临床杂志》2005年第1期18-20,共3页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的:观察exendin4对非糖尿病小鼠的血糖和糖耐量的影响。方法:非糖尿病小鼠分为exendin4(Exe4)各剂量组和生理盐水(NS)组。腹腔内注射Exe40.1μg·g-1,观察注射1h后、连续注射10d期间和停药后2wk内血糖,并于停药后20d行腹腔葡萄糖耐量试验(IPGTT)。另外,非糖尿病小鼠每日注射不同剂量Exe40.1,0.2,0.4μg·g-1×3d,观察每日血糖。结果:非糖尿病小鼠注射Exe40.1μg·g-11h后血糖为(6.2±s1.3)mmol·L-1,低于NS组(10.6±1.1)mmol·L-1,P<0.01。连续注射Exe4期间的平均血糖以及停药后2wk内的平均血糖与NS组比较,均无明显差异,P>0.05;且IPGTT中2组各点血糖差异无显著意义,不同剂量Exe4注射组小鼠的3d平均血糖与NS组比较也无明显差别。结论:Exe4对非糖尿病小鼠的血糖具有即刻降低作用,但是每日1次腹腔内注射对非糖尿病小鼠的血糖和糖耐量无明显影响。AIM: To investigate the effects of exendin-4 on glucose level and glucose tolerance in non-diabetic mice. METHODS: Non-diabetic mice were divided into exendin-4 and normal saline (NS) groups. After a bolus intraperitoneal administration of 0.1 μg·g -1 exendin-4, glucose levels were tested at 1 h after administration, followed by daily 10 d of injection and after the injection of 2 wk. Intraperitoneal glucose tolerance test (IPGTT) was performed on d 20 after the cessation. In addition, three different doses of exendin-4 (0.1, 0.2, 0.4 μg·g -1) were injected daily for 3 d and glucose levels were also tested daily during the injection. RESULTS: The glucose level of mice treated with 0.1 μg·g -1 exendin-4 1 h after the injection was significantly lower as comparing to NS treated mice, ((6.2±s 1.3) mmol·L -1 vs (10.6±1.1) mmol·L -1,P<0.01). The average glucose levels were not affected either during the injection or after the cessation. The results of IPGTT also showed no statistical difference between the two groups. Different doses of exendin-4 did not show notable difference in comparing to the NS group. CONCLUSION: The instant effect of exendin-4 on non-diabetic mice is to decrease glucose level, but daily injection shows no significant influence on glucose level and glucose tolerance.

关 键 词：糖尿病 降血糖药 小鼠 胰升糖素样肽-1 EXENDIN-4 

分 类 号：R977.15[医药卫生—药品]