ICI174,864对失血性休克大鼠全身及局部血管反应性变化的作用  

Changes in systemic and local vascular reactivity in hemorrhagic shock and the therapeutic effect of δ opioid receptor antagonist ICI174,864

作　　者：刘良明[1] 李萍[1] 刁有芳[1] 周学武[1] 周荣[1] 廖自福[1] 田昆仑[1] 刘建仓[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第二研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《中国危重病急救医学》2005年第1期16-19,共4页Chinese Critical Care Medicine

基　　金：国家自然科学基金资助项目(30271266;30370563)教育都回国人员启动基金国家"973"重点基础研究资助项目(G1999054202)

摘　　要：目的 观察大鼠不同程度失血性休克时全身和局部血管反应性变化及δ阿片受体特异性拮抗 剂ICI174,,864对失血性休克大鼠血管反应性的影响。方法56只Wistar大鼠,戊巴比妥钠麻醉(30 mg／kg)。 实验分两部分。第一部分实验用32只大鼠,随机分为手术对照组、1 h低血压组,2 h低血压组和3 h低血压 组.每组8只动物;动物经股动脉插管放血至血压40 mm Hg(1mm Hg=0.133 kPa)分别维持1、2和3 h,然 后回输全部残余失血。观察回输血后1、2和3 h血压和肠系膜上动脉(SMA)对去甲肾上腺素(NE.3μg／kg) 的反应性变化。第二部分实验用24只大鼠。随机分为失血性休克对照组、ICI174,864 0.5 mg／kg干预组和 1.0 mg／kg干预组,每组8只动物;动物经股动脉插管放血至血压40 mm Hg.维持2 h,回输残余失血,观察给 予ICI174.864后1、2和4 h血压和SMA对NE(3 μg／kg)的升压和收缩反应。结果 失血性休克后全身(血 压对NE的升压反应)和局部(SMA对NE的收缩反应)血管反应性显著降低,且呈一定的程度和时间依赖 性,即休克程度越重,时间越长,血管反应性降低越多。单纯回输失血不能纠正血管的低反应性,ICI174,864 对休克血管的低反应性有不同程度的恢复作用,且呈一定的剂量依赖关系。结论失血性休克可诱导全身和 局部血管反应性降低,并与休克程?Objective To investigate the changes in systemic and local vascular reactivity following hemorrhagic shock of different severity and the therapeutic effect of 8 opioid receptor antagonist ICI174.864. Methods Fifty - six Wistar rats were used in two experiments. In experiment Ⅰ , 32 rats were equally divided into sham operation group. 1 hour , 2 hours and 3 hours hypotension groups. In the latter groups, rats were bled to a mean arterial blood pressure(MAP) of 40 mm Hg(1 mm Hg =0.133 kPa) and maintained at this level for 1,2.3 hours, respectively. The pressor response of blood pressure and the contractile response of superior mesenteric artery (SMA) to norepinephrine (NE, 3 μg/kg) were observed after shed blood was reinfused. In experiment Ⅱ , 24 rats were divided into shock control > ICI174,864 0. 5 mg/kg and 1. 0 mg/kg groups. The response of blood pressure and SMA contractility to NE(3 pg/kg) were observed at 1, 2, and 4 hours after ICI174.864 administration. Results Following hemorrhagic shock, the systemic and local (SMA) vascular responsiveness was significantly decreased significantly and it was time dependent. Shed blood reinfusion alone did not restore the decreased vascular reactivity. ICI174.864 improved the decreased vascular reactivity in dose -dependent manner. Conclusion Hemorrhagic shock can induce systemic and local vascular hyporeactivity. The decreased vascular reactivity is closely associated with the severity and duration of shock. Loss of systemic vascular reactivity parallels to that of the regional vessel. 8 opioid receptor antagonist ICI174,864 has some beneficial effect in improving vascular hyporeactivity.

关 键 词：失血性休克 血管反应性 大鼠 全身 局部 血压 回输 和局 恢复作用 结论 

分 类 号：R605.971[医药卫生—急诊医学]