重组人白介素-2吸入给药对小鼠克雷伯肺炎感染的影响(英文)  

Effect of rhIL-2 in inhalant form on experimental respiratory tract infection with Klebsiella pneumoniae in mice

作　　者：汤玥[1] 朱家壁[1] 程度胜 张旭松[1] 周波[1] 申海燕[1] 

机构地区：[1]中国药科大学药物制剂研究所,江苏南京210009 [2]北京四环生物制药有限公司,北京100071

出　　处：《药学学报》2005年第1期17-21,共5页Acta Pharmaceutica Sinica

基　　金：SevereAcuteRespiratorySyndrome (SARS)ProjectY0308 supportedbyChinaPharmaceuticalUniversity.

摘　　要：目的　研究重组人白介素 2吸入给药对小鼠克雷伯肺炎感染的保护作用。方法　小鼠以滴鼻感染法进行感染。感染过程中,以鼻腔插管注入法和皮下注射给予小鼠重组人白介素 2。同时另设正常组、阴性对照组、模型组和阳性对照组(庆大霉素组)。观察各组动物肺病理变化,比较平均死亡时间和一周内死亡率,测定支气管肺泡洗涤液中总蛋白、白蛋白含量,碱性磷酸酶、乳酸脱氢酶活力。结果　重组人白介素 2给药组肺病理变化与模型组和阴性对照组相比显著减轻,支气管肺泡洗涤液中总蛋白、白蛋白含量和碱性磷酸酶活力降低。结论　吸入重组人白介素 2缓解感染后肺部病理变化, 为研发重组人白介素 2吸入剂提供了依据。Aim To determine the protective effect of recombinant human interleukin-2 (rhIL-2) in inhalant form on experimental respiratory tract infection with Klebsiella pneumoniae in mice. Methods Mice were infected with the method of nasal intubation drip. During infection, mice were given rhIL-2 by sc injection and the method of nasal intubation drip. There were normal group, vehicle group, model group, rhIL-2 groups and gentamicin group. In the end, the pathological changes in the lung were observed. The survival time and the mortality within a week of each group were recorded. The total protein content, the albumin content, the activity of alkaline phosphatase and the activity of lactic dehydrogenase of broncho-alveolar lavage fluid (BALF) were dertermined and compared. Results Symptoms of Klebsiella pneumoniae were remarkably relieved because of rhIL-2 administration. The total protein content, the albumin content, the activity of alkaline phosphatase and the activity of lactic dehydrogenase of BALF were less than those in the vehicle group and the model group. Conclusion Inhalation of rhIL-2 can alleviate the pathological changes in the lung after infection. At the same dose, it could be seen that the effect of rhIL-2 in inhalant form was better than that of the injection.

关 键 词：重组人白介素-2 吸入剂 克雷伯肺炎 感染 

分 类 号：R915[医药卫生—微生物与生化药学] R965[医药卫生—药学]