花椒及其混淆品的rDNA ITS区序列分析与鉴别  

Authentication of Zanthexylum bungeanum Maxin population and adulterants by analysis of rDNA ITS sequences

作　　者：沈洁[1] 丁小余[1] 张卫明[2] 保曙琳[1] 常俊[1] 唐凤[1] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物资源技术重点实验室,江苏南京210097 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《药学学报》2005年第1期80-86,共7页Acta Pharmaceutica Sinica

基　　金：国家"十五"攻关项目(2001BA502B05).

摘　　要：目的　研究不同居群的花椒及其混淆品的rDNAITS区碱基序列的特征及其差异,为花椒的鉴别提供可靠的分子标记。方法　运用PCR产物直接测序和克隆测序法对甘肃、陕西、四川、河北等 7个花椒居群及 3个混淆种的rDNAITS区(包括ITS1, 5 8S,ITS2)碱基序列进行序列测定。结果　首次报道花椒ITS区的碱基序列,序列总长度为 619-620bp,长度变异较少,与混淆种长度仅相差 4bp。花椒各居群中,rDNAITS区碱基序列有 15个变异位点、12个信息位点、3个特异性识别位点。与混淆品间的碱基差异则较为显著,多达 71个变异位点,有 4个花椒特异性识别位点。结论　依据花椒ITS区的序列特征可准确鉴别各居群的花椒及其混淆品;亲缘关系密切的花椒居群在地理位置上也非常靠近;rDNAITS序列特征可作为花椒种内和种间鉴别的有效分子标记。Aim To study the difference of rDNA ITS sequences between Zanthexylum bungeanum populations and their adulterants in main habitants of China so as to provide molecular markers for identifying Zanthexylum bungeanum populations against adulterants. Methods rDNA ITS regions (including ITS-1, 5.8S and ITS-2) of 7 populations of Zanthexylum bungeanum which are separate located in Gansu, Shanxi, Sichuan, Hebei provinces, and 3 adulterants were sequenced by PCR products sequencing method or clone sequencing method. Results The sequences of rDNA ITS region of Zanthexylum bungeanum were reported for the first time, and the sequences of ITS region ranged from 619 to 620 bp, and the length difference amoung Zanthexylum bungeanum and their adulterants is 4 bp. There are 15 variable sites, 12 informative sites and 3 authenticable sites among Zanthexylum bungeanum populations. The difference of rDNA ITS regions amoung Zanthexylum bungeanum and their adulterants is obvious, the number of variable sites is 71. Conclusion The difference of rDNA ITS sequences can be used to authenticate accurately the populations of Zanthexylum bungeanum and their adulterants. These populations of Z.bungeanum which have close relationship always distribute in near geographic areas. The characteristics of rDNA ITS sequence can be used as good markers for authenticating Zanthexylum bungeanum populations form their adulterants.

关 键 词：花椒 居群 混淆品 RDNA ITS区 DNA分子鉴别 

分 类 号：R282.5[医药卫生—中药学] R286[医药卫生—中医学]