乌鸡小脑的解剖学及神经肽Y神经元分布的研究  

Anatomy and Distribution of NPY Immunoreactive Neurons of the Cerebellum in Silky Fowl

作　　者：陈文钦[1] 彭克美[1] 刘华珍[1] 罗冠中[1] 

机构地区：[1]华中农业大学动物科技动物医学学院,湖北武汉430070

出　　处：《动物医学进展》2005年第1期51-54,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金资助项目 ( 3 9970 5 47)

摘　　要：为了探明乌鸡小脑的形态结构及其细胞构筑和神经肽Y免疫反应神经元在小脑内的分布情况 ,采用石蜡切片HE染色和免疫组化SABC染色技术 ,对 1 0只泰和乌鸡的小脑进行了研究 ,并与北京鸭、肉鸽、本地鸡及非洲鸵鸟等禽类的相关结构进行了比较。结果表明 :①泰和乌鸡小脑皮质分 3层 ,由外至内依次为分子层、蒲肯野氏细胞层和颗粒层 ;②小脑分 1 0叶 ,Ⅰ、Ⅱ和Ⅹ为单叶 ,其余都有2个～ 3个小叶 ;③小脑中央核的神经元数量稀少 ;④神经肽Y免疫阳性神经元主要存在小脑皮质的蒲肯野氏细胞层 ,分子层、颗粒层 ,在小脑中央核未见阳性反应细胞 ;⑤泰和乌鸡小脑的形态结构及其细胞构筑与其它家禽相似 ;⑥小脑内NPY阳性神经元在蒲肯野氏细胞层的分布与鸡、肉鸽、大鼠的大体相似 。In order to investigate the cytoarchitecture and localization of neuropeptide Y(NPY) immunoreactive neurons in silky fowl cerebellum, the cerebellums of ten silky fowls were studied by paraffin consecutive sections, HE and the immunohistochemical SABC staining method (StreptAvidin-Biotin complex) and compared with that of Pekin, meat pigeon, local chick, African ostrich. Cytoarchitecture of the cerebellar cortex and central nuclei, and then the distribution of neuropeptide Y immunoreactive neurons were observed under the light microscope. The results show as follows:①there are three layers in the cerebellar cortex of silky fowl including molecular layer,Purkinye cell layer and granular layer from outside to inside;②there are ten lobules in cerebellum including two or three cerebellar folia every lobule, except thatⅠ,Ⅱand Ⅹis only one; ③there are a few neurons in cerebellar central nuclei;④immunoreactive neurons of NPY in silky fowl cerebellum are mainly located in the Purkinje cell layer of the cerebellar cortex, which is not found in molecular layer, granular layer and cerebellar central nuclei.The results of the present study indicate that morphology and cytoarchitecture of the cerebellum in silky fowl is similar to other domestic fowls; the localization of neuropeptide Y immunoreactive neurons in silky fowl cerebellar cortex is similar to that of chick, meat pigeon and rat, but, in cerebellar white matter is different from that of meat pigeon.

关 键 词：细胞构筑 神经肽Y 免疫组织化学 小脑 乌鸡 

分 类 号：S852.162[农业科学—基础兽医学] S858.31[农业科学—兽医学]