广西巴马小型猪IFN-Y基因的克隆和序列分析  

Cloning and Sequencing of Y -Interferon Gene of Bama mini pig

作　　者：韦祖樟[1] 黄伟坚[1] 卢桂娟[1] 孙文博[1] 颜建华[1] 罗廷荣[1] 陆芹章[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005

出　　处：《动物医学进展》2005年第1期59-61,共3页Progress In Veterinary Medicine

基　　金：广西大学科学技术研究重点基金资助项目 ( 2 0 0 3ZD0 1) ;广西科技攻关项目 (桂科攻 0 480 0 0 1)

摘　　要：将猪外周淋巴细胞进行体外培养 ,在刀豆球蛋白A(conA)刺激培养 1 5h后 ,提取培养淋巴细胞总RNA ,应用RT PCR技术扩增广西巴马小型猪Y 干扰素 (IFN Y)基因 ,并克隆到PMD1 8 T载体上 ,进行测序。测序结果表明 ,扩增的cDNA全长 558个碱基 ,包含 50 1个碱基开放阅读框架 ,编码 1 66个氨基酸 ,分子量为 1 9.42ku。此cDNA与人、牛、山羊、马、狗和鼠的IFN Y基因核苷酸比较 ,同源性分别为 74.9% ,86.5% ,86.9% ,81 % ,58.7%和 63 .3 % ,与其它猪种的IFN Y基因核苷酸比较同源性为 1 0 0 %。The porcine peripheral blood lymphocytes were cultured in vitro. After ConA was used to stimulate the lymphocytes for 15h, total RNA was isolated from the lymphocytes.the mini porcine IFN-Y gene was amplified by reverse transcription polymerase chain reaction (RT-PCR). PCR product was cloned into PMD18-T vector. Consequently, a DNA clone was obtained encoding Bama mini pig IFN-Y of 558 bp in length, which contained a complete open reading frame of 501 bp. Bama mini porcine IFN-Y was composed of 166 amino acids, which predicted molecular weight of 19 420. Bama mini porcine IFN-Y has an amino acid homology of 74.9% with human IFN-Y, 86.5% with bovine, 86.9% with goat,81% with horse, 58.7% with dog and 63.3% with rat. The homology of IFN-Y was 100% at nucleotide level between Bama mini pig and other pigs.

关 键 词：巴马小型猪 Y-干扰素基因 克隆 测序 

分 类 号：Q78[生物学—分子生物学] S858.28[农业科学—临床兽医学]