嗜盐古生菌AJ6的分离及系统发育分析  

Isolation and phylogenetic analysis of halophilic archaea AJ6.

作　　者：徐晓红[1] 吴敏[1] 曹轶[1] 吴月红[1] 张婷[1] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310027

出　　处：《浙江大学学报（理学版）》2005年第1期83-88,共6页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金资助项目(30370029).

摘　　要：从新疆阿尔金山国家自然保护区阿牙克库木湖分离纯化得到嗜盐古生菌AJ6,革兰氏染色呈阴性、杆状、少数球形、菌体大小为0.2～0.6μm×1.6～4.2μm,最适NaCl和Mg2+质量浓度分别为15%和0.6%,在pH为6.0～7.0条件下生长良好.在此基础上,进一步通过PCR方法扩增了菌株AJ6的16SrRNA基因(16SrDNA),测定了该基因的核苷酸序列,并利用"Clustalw"软件和"PHYLIP"软件包分析16SrDNA序列,对其进行同源性比较和系统发育学分析,建立了嗜盐古细菌的系统发育树.结果表明菌株AJ6与Nnm.pallidum、Nnm.pellirubrum和Nnm.versiforme分在同一类群中,16SrDNA序列同源性分别为97.47%,96.44%和98.12%,菌株AJ6是Natrinema属中的一个新成员,命名为Natrinemaaltuensis.菌株AJ6的16SrDNA序列已登陆到GenBank,其序列号为AY277584.Halophilic Archaea AJ6 was isolated and purified from the Altun Mountain National Nature Reserve of Xinjiang Uygur Autonomous Region. Strain AJ6 is Gram-negative rods, a few are globular, with the size of single cell of 0.2-0.6 μm by 1.6-4.2 μm. The optimum salt concentration for its growth is 20% NaCl and 0.6% Mg^(2+), and the optimum pH is 6.0-7.0. Based on its morphology and physiological and biochemical features, 16S rRNA encoding gene (16S rDNA) from AJ6 was amplified by PCR, and their nucleotide sequences were determined. 'Clustalw' and 'PHYLIP' software bags were used to analyze 16S rDNA sequence, and the homology was compared and phylogenetic analysis was exerted, at the same time, phylogenetic tree of halophilic archaea was established. It was considered that strain AJ6 is a novel species of the genus Natrinema, and the name Natrinema altuensis is proposed. The GenBank accession numbers of the 16S rDNA sequences about strain AJ6 is AY277584.

关 键 词：新疆阿尔金山盐湖地区 嗜盐古生菌 16S RDNA 系统发育 

分 类 号：Q939[生物学—微生物学]