成年SD大鼠嗅球中几种蛋白的免疫组化分布研究  

Immunohistochemical localization of several proteins in the olfactory bulb of adult sprague dawley rat.

作　　者：秦照萍[1] 叶树明[1] 杜继曾[1] 沈公羽[1] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310027

出　　处：《浙江大学学报（理学版）》2005年第1期94-98,共5页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金资助项目(30070190).

摘　　要：对成年SD大鼠嗅球中tyrosinehydroxylase、calbindinD-28K、calretinin、parvalbumin及olfactorymarkerprotein几种蛋白进行了免疫组化分布的研究.结果表明:tyrosinehydroxylase主要出现在嗅球的小球层,在外网织层有很少的阳性细胞;calbindinD-28K主要出现在小球层,在外网织层和内网织层很少;calretinin在小球层、外网织层、僧帽细胞层、内网织层、粒细胞层都有分布,嗅神经层无阳性;parvalbumin在外网织层最明显,僧帽细胞层中有少量,在小球层很少;olfactorymarkerprotein只出现在嗅神经层和小球层.此研究结果与前人的相关研究结果基本接近,但也存在一些差异.The immunohistochemical localization of tyrosine hydroxylase, calbindin D-28K, calretinin, parvalbumin and olfactory marker protein in olfactory bulb of adult Sprague Dawley rat was studied. Tyrosine hydroxylase are mainly found in glomerular layer,few in external plexiform layer. Calbindin D-28K is mainly found in glomerular layer, few in external plexiform layer and internal plexiform layer. Calretinin is found in glomerular layer, external plexiform layer, mitral cell layer, internal plexiform layer and granule cell layer except olfactory nerve layer. Parvalbumin is mostly found in external plexiform layer, some in mitral cell layer, few in glomerular layer. Olfactory marker protein is found only in olfactory nerve layer and glomerular layer. Results are similar to the pioneering research in this field, but some difference exists.

关 键 词：成年SD大鼠 嗅球 tyrosine hydroxylase CALBINDIN D-28K CALRETININ PARVALBUMIN olfactory marker protein 免疫组化 

分 类 号：Q954.52[生物学—动物学] Q954.62