必需脂肪酸对大鼠脑组织中EAA含量的影响  

Effects of essential fatty acids on excitatory amino acid in rat brain

作　　者：张侃[1] 曾琳[2] 龙在云[2] 刘媛[2] 李应玉[2] 杨恒文[2] 伍亚民[2] 陶翠兰[2] 

机构地区：[1]重庆医科大学附属儿童医院儿保教研室,重庆400014 [2]第三军医大学大坪医院野战外科研究所第三研究室,重庆400042

出　　处：《第三军医大学学报》2005年第1期53-55,共3页Journal of Third Military Medical University

摘　　要：目的　探讨在大鼠脑发育期补充必需脂肪酸兴奋性氨基酸 (EFA)对脑组织中EAA含量的影响。方法　断乳大鼠 (1个月 ) 3 0只 ,雌雄不拘 ,分为正常组、EFA缺乏组和补充鱼油组 ,分别以不同饲料喂养 3个月 ,取出新鲜的大脑皮层和海马组织 ,测定天门冬氨酸、谷氨酸的含量 ;尼氏染色和嗜银染色观察大脑皮质和海马细胞形态结构的变化。结果　与正常组相比 ,EFA缺乏组的EAA含量均显著升高 (P <0 0 5 ) ,补充鱼油组无明显差异 ;与补充组相比 ,EFA缺乏组大脑皮质大锥体细胞数目明显减少、排列不规则、稀疏、轴突纤维短 ,海马细胞层数较少、体积也较小。结论　发育期EFA缺乏会导致脑组织中EAA含量明显升高 ,神经形态异常。Objective To investigate the effects of essential fatty acids (EFA) on the excitatory amino acid in tissues of rat brains. Methods Ninety neonatal rats, one-month old, were randomly divided into normal, EFA deficiency and fish oil supplementation groups and were fed with different forages for three months, respectively. The contents of aspartic acid and glutamic acid in the cortex and hippocampus were determined. The changes of morphology and structure of the cortex and hippocampus were observed by Nissl and silver staining. Results Compared with those in the normal group, the EAA contents in EFA deficiency group were significantly increased (P<0.05), but in fish oil supplementation group, no significant changes were observed. The number of large pyramidal cells in the cortex was much less than that in the fish oil supplementation group. At the same time, the length of axons was shorter in deficiency group. In the hippocampus, the layer and the size of cells were less than those in the fish oil supplementation groups. Conclusion Deficiency of EFA at the developmental period can increase the EAA contents in brain and cause abnormality of neural morphology.

关 键 词：必需脂肪酸 兴奋性氨基酸 脑组织 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R339.3[医药卫生—基础医学]