丝素改性胶原膜的肝素化及其体外抗凝血性能评价  

Immobilization of heparin to silk fibroin/collagen blend films and in vitro antithrombogenicity assessment

作　　者：冯桂龙[1] 王松[2] 朱鹤孙[2] 

机构地区：[1]湖北襄樊学院化学与生物科学系,湖北襄樊441053 [2]北京理工大学材料科学研究中心,北京100081

出　　处：《功能材料》2005年第1期150-152,156,共4页Journal of Functional Materials

基　　金：国家重点基础研究发展规划项目资助(G1999064705)

摘　　要：以1- (3- 二甲氨基丙基)- 3 -乙基碳二亚胺(简称EDCI)为缩合剂对丝素改性胶原膜进行了肝素固定化,测定并研究了固定化肝素的稳定性,应用 X 光电子能谱分析了材料的表面性质。以凝血酶原时间(PT)、部分凝血活酶时间(APTT)、凝血酶时间(TT)等3个体外凝血时间为评价标准,考察了材料的抗凝血性能。结果表明,肝素化后的丝素改性胶原膜具有良好的抗凝血性。The Silk Fibroin/Collagen blend film was heparinized in the presence of 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide (EDCI). X-ray photoelectron spectroscopy (XPS) was employed to analyze the surface of films. The antithrombogenicity was tested by prothrombin time (PT), activated partial thromoplatin time (APTT) and thrombin time (TT), which were in vitro coagulation times. The results showed that most heparins were covalently bound to the functional groups of blend film and the heparinized film had high antithrombogenicity.

关 键 词：丝素 胶原 肝素化 抗凝血性 

分 类 号：O632.31[理学—高分子化学] R973.2[理学—化学]