银杏GinNdly基因的植物表达载体构建  

Construction of plant expression vecotors containing GinNdly gene

作　　者：张建业[1,2] 陈力耕[1] 姜国良[3] 

机构地区：[1]浙江大学园艺系 [2]中国海洋大学海洋生命学院,山东青岛266003 [3]中国海洋大学海洋生命学院

出　　处：《果树学报》2005年第1期16-18,共3页Journal of Fruit Science

基　　金：国家自然科学基金资助项目(30070634)。

摘　　要：未知功能的植物基因一般需要通过转基因植物来研究和验证。银杏(GinkgobilobaL.)是一种童期很长的古老植物。LEAFY基因是一个花分生组织特征基因,调控着植物开花的时间。用植物双元表达载体质粒pCAMBI-A1301构建了开花基因LEAFY的银杏同源基因GinNdly的反义与正义植物表达载体。因pCAMBIA1301质粒的多克隆位点处没有启动子和终止子,将pBI121的35S启动子和nos终止子引入该质粒。通过PCR检测和酶切验证,证明质粒构建正确,为研究银杏花分生组织特征基因GinNdly奠定了基础。To study the function of a unknown gene, a sense plant expression vectors and an antisense plant expression vectors were constructed by fusing a LEAFY(LFY)-like GinNdly with pCAMBIA1301. GinNdly was cloned from a male tree of Ginkgo biloba L. whose juvenile period is very long. LEAFY(LFY), one of floral meristem identity genes, controls transition from vegetative to floral meristem identity in Arabidopsis, and affects its flowering time. Before constructing expression vectors, pCAMBIA1301 which has no a promoter and a terminator was loaded with a promoter of 35 S and terminator of NOS which were derived from a plasmid pBI121 to form a plasmid of pCAMBIA1301-35 S-NOS. The two vectors were detected by PCR and digested with restriction endonucleases and were proved right. This gave a start of studying floral meristem identity genes in Gingko biloba.

关 键 词：银杏 GinNdly 植物表达载体 构建 

分 类 号：S664.3[农业科学—果树学]