Eotaxin基因在真菌诱导的变应性鼻炎鼻黏膜中的表达及其意义  

Expression of Eotaxin gene in nasal mucosa of allergic rhinitis in the guinea pig sensitized by fungal allergen

作　　者：张志明[1] 孙树岩[2] 王寿忱[3] 季文樾[1] 关超[1] 

机构地区：[1]中国医科大学附属第一医院耳鼻咽喉科 [2]解放军第202医院耳鼻咽喉-头颈外科 [3]牡丹江医学院红旗医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2005年第2期78-81,共4页Journal of Clinical Otorhinolaryngology

摘　　要：目的:探讨真菌诱导的豚鼠变应性鼻炎鼻黏膜中Eotaxin基因的表达及其意义。方法:采用真菌互 隔交链孢霉致敏豚鼠建立变应性鼻炎动物模型,用组织病理学方法观察鼻黏膜改变,原位杂交方法检测鼻黏膜中 Eotaxin基因的表达情况。结果:行为学打分,模型组明显高于对照组;鼻黏膜固有层中见大量嗜酸粒细胞浸润; 模型组鼻黏膜中Eotaxin基因的表达主要在黏膜固有层,对照组未见明显阳性表达。结论:真菌诱导的变应性鼻 炎鼻黏膜中Eotaxin基因表达明显增强,大量嗜酸粒细胞浸润,进而引起组织损伤,表明Eotaxin基因在真菌诱导 的变应性鼻炎病理过程中起重要作用。Objective:To study the expression and significance of Eotaxin gene in nasal mucosa of guinea pig sensitized by fungal allergen.Method:Alternaria alternata sensitized guinea pigs used as animal model of allergic rhinitis(AR).The changes in the nasal mucosa were studied by pathological method and the Eotaxin gene were studied by In Situ Hybridization.Result:The behavior science score of experimental group was significant higher than the controls group.Plenty eosinophils were found in lamina propria of nasal mucosa of experimental group. Eotaxin gene is expressed in lamina propria of nasal mucosa of experimental group.The controls group had not positive expression.Conclusion:Eotaxin gene is expressed at high levels in nasal mucosa of fungal allergic rhinitis in the guinea pig.Plenty eosinophils were found in nasal muscosa.We consided that Eotaxin gene and eosinophils could play a key role in fungal allergic rhinitis.

关 键 词：鼻炎 变应性 基因 eotaxin 真菌 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]