鹦鹉热衣原体重组主要外膜蛋白诱导肉鸡免疫应答观察  

Induction of immune responses in broiler by recombinant major outer membrane protein of Chlamydia psittaci

作　　者：郝永新[1] 何诚[1] 赵德明[1] 武艳[1] 朱虹[2] 端青[2] 

机构地区：[1]中国农业大学实验动物研究所,北京100094 [2]军事医学科学院微生物流行病研究所

出　　处：《中国人兽共患病杂志》2004年第12期1065-1067,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目 (3 0 3 70 0 70 )

摘　　要：用鹦鹉热衣原体 (Chlamydiapsittaci,Cps)重组主要外膜蛋白 (RecombinentMajorOuter Membraneprotein ,r MOMP)制作成油佐剂疫苗 ,经颈部皮下免疫 5d肉鸡。在免疫前 1d、免疫后 14d、2 1d和 30d分别翅静脉采血 ,以常规血凝抑制试验检测抗体效价 ;用MTT法检测全血淋巴细胞对r MOMP的特异性增殖反应 ;免疫 30d使用鹦鹉热衣原体强毒株(BJF5株 )攻毒 ,观察临床症状 ,计算病变程度和保护率。结果表明免疫 14d后产生抗体 ,效价达 4 .2 (log2 ) ,免疫后 30d效价达到 5 .0 (log2 ) ;免疫组血液T淋巴细胞对r MOMP的增殖指数明显高于对照组 (P <0 .0 5 )。试验表明r MOMP在肉鸡体内可诱导对Cps特异性的体液免疫和细胞免疫应答 ,并能抵抗Cps强毒的攻击 ,显示r MOMP免疫肉鸡具有良好的免疫原性和保护性。The 5 days old broilers were inoculated with recombinant major outer membrane protein(rMOMP) vaccine of Chlamydia psittaci (Cps) subcutaneously in the cervical region, sera were taken from each broiler on day 1, 14, 21 and 30 after inoculation, antibodies against rMOMP were determined by indirect hemoagglutinatie inhibition test, and the proliferation of the T lymphocytes in blood after stimulation with antigen rMOMP was investigated with MTT assay. On day 30, birds were challenged with vivid Cps, and the pathological changes and the protective rates were evaluated.The results demonstrated that the antibody titers reached up to 4.2(log 2) on day 14, and 5.0(log 2) on day 28. The stimulation index in proliferative response to rMOMP was significantly higher than that of the controls. It is concluded that immunization with rMOMP can generate both the humoral and cellular immune responses in broilers.

关 键 词：鹦鹉热衣原体 重组主要外膜蛋白 肉鸡 免疫应答 

分 类 号：S852.67[农业科学—基础兽医学]