复方氯胺酮口服液对鼠小脑N-甲基D-天冬氨酸受体1和γ氨基丁酸A受体的影响  

Effect of compound ketamine oral solution on NMDAR1 and GABAAR in Purkinje neurons

作　　者：张锦[1] 汪薇[2] 邵素娟 

机构地区：[1]中国医科大学附属第二医院麻醉科,辽宁沈阳110004 [2]中国医科大学基础医学院设备处 [3]盘锦市第四人民医院麻醉科

出　　处：《中国医科大学学报》2004年第6期481-483,共3页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20022074)

摘　　要：目的观察大鼠服用复方氯胺酮口服液(CKOS)后不同时点行为变化及小脑中N甲基D天冬氨酸受体1(NMDAR1)和γ氨基丁酸A受体(GABAAR)的分布,探讨其口服液对鼠小脑NMDAR1和GABAAR的影响及其作用机制。方法采用免疫组化、原位杂交技术检测SD大鼠服用CKOS后不同时点NMDAR1和GABAAR在小脑Purkinje细胞的表达情况。结果0点处(正常对照)NMDAR1在小脑Purkinje细胞表达最多;随着药效的增加,NMDAR1在小脑Purkinje细胞上的表达逐渐减少,30min时NMDAR1表达最低值,120min时Purkinje阳性细胞逐渐恢复,360min恢复接近正常。而GABAAR与NMDAR1的表达方式正相反,GABAAR在Prukinje细胞上的表达随着药效的增强而增加,又随着药效减低而减少。结论CKOS对鼠小脑NMDAR1在Purkinje细胞上的表达呈剂量依赖抑制,应产生不协调运动。但GABAAR在Purkinje细胞上的表达呈剂量依赖增强,抑制不协调运动。CKOS对小脑的作用为促使大鼠协调运动。Objective: To observe the effect of compound ketamine oral solution on N-methyl-D-aspartate receptor1 ( NMDAR 1) and gamma-aminobutyric acid A receptors (GABAAR ) in Purkinje neurons of mice. Methods: Immune histochemistry staining and in situ hybridization were used to check the distribution of NMDAR 1 and GABA AR in Purkinje neurons of mice. Results: Both GABAAR and NMDAR 1 all showed on cerebellar Purkinje neurons. The expression of NMDAR 1 at the first time point were significantly more than that at other time points (P<0.01). Plasma of cell were stained as brown. The expression of GABAAR at zero time point was lower than that at other parts in cerebellar Purkinje neurons. The expressions of NMDAR 1 became weaker gradually after the first time point; However, the expression of GABAAR became stronger with onset of compound ketamine oral solution. Conclusion: Compound ketamine oral solution can slightly increase the expression of GABAAR and sharply decrease the expression of NMDAR 1 in cerebellar Purkinje neurons, and improve the harmony movement of the mice.

关 键 词：复方氯胺酮口服液 N-甲基-D-天冬氨酸受体1 γ氨基丁酸A受体 小脑Purkinje细胞 大鼠 

分 类 号：R614[医药卫生—麻醉学]