李氏5号方对Aβ导致神经元毒性的保护作用  

Lishi No.5 Formula Capsule on Neurotoxicity Induced by Aβ

作　　者：张景艳[1] 王蓉[1] 李子中 姬志娟[1] 程浩然 李生海 盛树力[1] 

机构地区：[1]首都医科大学宣武医院北京脑老化重点实验室 [2]广东省高明脑病医疗医药研究院

出　　处：《首都医科大学学报》2004年第4期472-474,共3页Journal of Capital Medical University

摘　　要：观察李氏 5号方对人神经母细胞瘤株SY5Y的细胞生长情况和对 β 淀粉样肽 (β amyloid ,Aβ)导致神经毒性的影响 ,从而在细胞水平上证实该中药对神经细胞的保护作用。用固相法合成Aβ2 5 35,高效液相纯化 ,李氏 5号方水提液 0 .5 g/mL。将人神经母细胞瘤株SY5Y细胞接种后分为正常对照组、Aβ2 5 352 5 μmol/L损伤组和Aβ2 5 352 5μmol/L加李氏 5号方水提液保护组。以噻唑蓝 (MTT)代谢率、乳酸脱氢酶 (LDH)漏出率、细胞轴突长度、胞体面积为观察指标。结果 :与正常对照组相比 ,Aβ2 5 35组SY5Y细胞的轴突长度和胞体面积均较小 ,MTT代谢率降低 ,LDH漏出率升高 ,而加入李氏 5号方保护后 ,可使上述指标恢复或接近正常。提示 :李氏 5号方具有神经保护作用 ,可减轻Aβ导致的神经毒性。The aim was to observe the the effect of Lishi No.5 Formula capsule on the growth of human neuroblastoma cell line SY5Y and on the neurotoxicity induced by Aβ. Aβ 25-35 was synthesized by solid phase method and purified by HPLC. Lishi No.5 Formula water extract was 0.5 g/mL. Human neuroblastoma cells SY5Y were segregated into 3 groups: normal control group; Aβ 25-35(25 μmol/L) damaged group; and Aβ 25-35(25 μmol/L) + Lishi No.5 Formula water extract (corresponding to Lishi No.5 Formula 0.5 g/L culture medium) for neuroprotection group. MTT metabolic rate, LDH leakage rate, axonal length, area of cell body were used as indicators. Compare with normal control group, Aβ 25-35 damaged group showed reduced MTT metabolic rate, increased LDH leakage rate, and diminished axonal length and area of cell body, but the addition of Lishi No.5 Formula normalized the foregoing changes. Lishi No.5 Formula has neuroprotective effect. It can diminish neurotoxicity of Aβ.

关 键 词：AΒ 正常 保护作用 水提液 对照组 神经母细胞瘤株 神经毒性 代谢率 乳酸脱氢酶(LDH) 轴突 

分 类 号：R285.5[医药卫生—中药学] R749.16[医药卫生—中医学]