毛细管电泳化学发光法用于铁形态的分析  

Study on the Capillary Electrophoresis with Chemiluminescence Detection and the Analysis of the Species of Iron

作　　者：卫洪清[1] 刘二保[1] 梁建功[2] 程介克[2] 

机构地区：[1]山西师范大学化学与材料科学学院,山西临汾041004 [2]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《山西师范大学学报（自然科学版）》2004年第4期74-78,共5页Journal of Shanxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(编号:20075017);山西省自然科学基金(编号:20021022).

摘　　要：以邻菲咯啉为配位剂,将毛细管电泳与鲁米诺-过氧化氢化学发光体系结合,成功的分离并检测了Fe(II)和Fe(III).在该体系中引入邻菲罗啉有以下优点:(1)有效的防止了Fe(II)被空气氧化的可能性.(2)提高了Fe(II)检测灵敏度.(3)提高了Fe(II)和Fe(III)的分离效率.在pH4.3的醋酸-醋酸钠缓冲溶液中,不到8min的时间即可实现Fe(II)和Fe(III)的分离.此法对Fe(II)检测的线性范围是1nmol/L～200nmol/L,对Fe(III)的线性范围是10nmol/L～330nmol/L,它们的检出限分别是Fe(II)68amol(3.4×10-9mol/L)和Fe(III)460amol(2.3×10-8mol/L).A new method for the simultaneous determination of iron(II) and iron(III) using the chelating reagent 1,10-phenanthroline is firstly reported. Capillary electrophoresis (CE) with chemiluminescence (CL) detection was used in the separation of iron(II) and iron(III) complexes which catalyze the CL reaction of luminol with hydrogen peroxide (H2O2). It was found that 1,10-phenanthroline could improve the separation efficiency of iron(II) and iron(III) and markedly enhance the chemilumiescence signal of iron(II), and meanwhile avoid that iron(II) is rapidly oxidized by oxygen. The metal ions were separated in less than 8 min with sodium acetate buffer (pH=4.3). The method was linear for iron(II) in the range 1nmol/L to 200nmol/L and for iron(III) from 10nmol/L to 330nmol/L. The procedure has detection limits of 68 amol (3.4×10-9 mol/L) and 460 amol (2.3×10-8 mol/L) for iron(II) and iron(III), respectively.

关 键 词：Fe(Ⅱ) Fe(Ⅲ) 邻菲咯啉 毛细管电泳 配位 鲁米诺 化学发光体系 化学发光法 分析 防止 

分 类 号：O657[理学—分析化学] O614.811[理学—化学]