微流控芯片系统流式细胞术及单细胞荧光检测  

Flow Cytometry and Single Cell Fluorescence Detection on a Microfluidic Device

作　　者：姚波[1] 冯雪[1] 罗国安[1] 王义明[1] 

机构地区：[1]清华大学化学系,北京100084

出　　处：《高等学校化学学报》2005年第1期43-45,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金重大项目 (批准号 :2 0 2 990 3 0 6)资助

摘　　要：A microfluidic device was developed for flow cytometry and single cell fluorescence detection. TO-PRO-3 as a vital DNA dye was used to label dead cells and some of apoptosis cells which could be penetrated from their broken membranes and intercalated into DNA chains. Cells were driven by combined effect of hydrodynamic and electric power and passed through the detection point one by one in micro channels. A laser induced fluorescence detection system which employed a 635 nm semiconductor laser was the only measurement of the whole system. This micro flow cytometor system was applied to estimate the damage degree of those HeLa cells having been exposed to ultraviolet radiation for 10, 20 and 40 min, respectively by the number of cells determined and their fluorescence intensity.A microfluidic device was developed for flow cytometry and single cell fluorescence detection. TO-PRO-3 as a vital DNA dye was used to label dead cells and some of apoptosis cells which could be penetrated from their broken membranes and intercalated into DNA chains. Cells were driven by combined effect of hydrodynamic and electric power and passed through the detection point one by one in micro channels. A laser induced fluorescence detection system which employed a 635 nm semiconductor laser was the only measurement of the whole system. This micro flow cytometor system was applied to estimate the damage degree of those HeLa cells having been exposed to ultraviolet radiation for 10, 20 and 40 min, respectively by the number of cells determined and their fluorescence intensity.

关 键 词：微流控芯片 流式细胞术 单细胞分析 激光诱导荧光 TO-PRO-3 

分 类 号：O657[理学—分析化学]